|
| Status |
Public on Jun 27, 2011 |
| Title |
The small RNA PhrS stimulates synthesis of the Pseudomonas aeruginosa quinolone signal |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
Quorum sensing, a cell-to-cell communication system based on small signal molecules, is employed by the human pathogen Pseudomonas aeruginosa to regulate virulence and biofilm development. Moreover, regulation by small trans-encoded RNAs has become a focal issue in virulence gene expression of bacterial pathogens. In this study, we have identified the small RNA PhrS as an activator of PqsR synthesis, one of the key quorum sensing regulators in P. aeruginosa. Genetic studies revealed a novel mode of regulation by a sRNA, whereby PhrS uses a base-pairing mechanism to activate a short upstream open reading frame to which the pqsR gene is translationally coupled. Expression of phrS is induced by the oxygen-responsive regulator ANR when the oxygen supply decreases. Thus, PhrS is the first bacterial sRNA that provides a regulatory link between oxygen availability and quorum sensing, which may impact on oxygen-limited growth in P. aeruginosa biofilms.
Keywords: genetic modification
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| |
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| Overall design |
Comparative transcriptome analysis with the delta-phrS strains PAO6671(pJT19) and PAO6671(pJTphrS), whereby the first strain harboured the parental vector and the latter a plasmid-borne inducible phrS gene
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| |
|
| Contributor(s) |
Sonnleitner E, Gonzalez N, Haas D, Blaesi U |
| Citation(s) |
21375594 |
| Submission date |
Nov 24, 2010 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Sylvain Pradervand |
| E-mail(s) |
Sylvain.Pradervand@unil.ch
|
| Phone |
+41 21 692 39 08
|
| Organization name |
UNI Lausanne
|
| Department |
CIG
|
| Lab |
DNA Array Facility
|
| Street address |
Genopode
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
|
| Samples (4)
|
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| Relations |
| BioProject |
PRJNA134237 |
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