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| Status |
Public on Feb 19, 2024 |
| Title |
Multiplex single cell analysis of serotonergic neuron function in planarians reveals widespread effects in diverse cell types |
| Organism |
Schmidtea mediterranea |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used scRNA-seq to examine serotonergic function in asexual planarians. We profiled 47,292 single cell transcriptomes of planarian of 3 different RNAi categories: gfp(RNAi), lhx1/5-1(RNAi) and pitx(RNAi). We detected changes in cell type frequency and gene expression patterns in planarian of different knock-down conditions, capturing regulatory programs of distinct cell types in response to loss of serotonergic function.
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| Overall design |
We obtained cell dissociations from adult populations of the asexual strain of Schmidtea mediterranea of serotonergic trascription factors (pitx and lhx1/5-1) RNAi conditions.
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| Contributor(s) |
Emili E, Rodríguez-Fernández D, Pérez-Posada A, García-Castro H, Solana J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 18, 2024 |
| Last update date |
Feb 19, 2024 |
| Contact name |
Jordi Solana |
| E-mail(s) |
scbe.lab@gmail.com
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| Organization name |
University of Exeter
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| Department |
Health and Life Sciences
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| Street address |
Northcote House
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| City |
Exeter |
| ZIP/Postal code |
EX4 4QD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL29967 |
Illumina NovaSeq 6000 (Schmidtea mediterranea) |
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| Samples (5)
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| GSM8084430 |
Schmidtea mediterranea, whole-body, scRNA-seq, batch 1, library 43_1_shallow, library 43_1_deep |
| GSM8084431 |
Schmidtea mediterranea, whole-body, scRNA-seq, batch 2, library 43_2_shallow, library 43_2_deep |
| GSM8084432 |
Schmidtea mediterranea, whole-body, scRNA-seq, batch 3, library 43_3_shallow, library 43_3_deep |
| GSM8084433 |
Schmidtea mediterranea, whole-body, scRNA-seq, batch 4, library 43_4_shallow, library 43_4_deep |
| GSM8084434 |
Schmidtea mediterranea, whole-body, scRNA-seq, batch 5, library 43_5_shallow, library 43_5_deep |
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| Relations |
| BioProject |
PRJNA1077699 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE256032_barcodes.tsv.gz |
594.4 Kb |
(ftp)(http) |
TSV |
| GSE256032_features.tsv.gz |
231.5 Kb |
(ftp)(http) |
TSV |
| GSE256032_matrix.mtx.gz |
39.9 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
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