NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE256261 Query DataSets for GSE256261
Status Public on May 14, 2024
Title Reanalysis of white and brite adipocyte ChIP-Seq of PPARG and MED1 in the hg38 genome
Sample organism Homo sapiens
Experiment type Third-party reanalysis
Genome binding/occupancy profiling by high throughput sequencing
Summary To compare the binding of PPARG and MED1 at the TSSes of differentially expressed isoforms in white and brite (also known as beige) adipocytes, data from GSE59703 (Loft et al., 2015) was reanalysed on the hg38 genome. We find that a large majority of the splice variants arise from differential TSS usage, with beige-specific TSSs being enriched for PPARĪ³ and MED1 binding compared to white-specific TSSs.
 
Overall design PPARgamma, MED1 ChIP-Seq and Input reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). These consisted of two replicates per antibody in each condition and an input file per condition to a total of 10 samples. Reads from replicates were merged and aligned to hg38 using Bowtie2. Peaks were detected using MACS3. ChIP/Input ratio tracks were generated using bamCompare in deepTools. Mean ratios (ChIP/Input) of the PPARgamma and MED1 were calculated for +/- 250 bp windows around the TSS using multiBigwigSummary from deepTools.
 
Contributor(s) Hazell Pickering S, Abdelhalim M, Collas P, Briand N
Citation Sarah Hazell Pickering, Mohamed Abdelhalim, Philippe Collas, and Nolwenn Briand. Alternative isoform expression of key thermogenic genes in human beige adipocytes. Front Endocrinol, Vol 15, 2024. doi:10.3389/fendo.2024.1395750
Submission date Feb 21, 2024
Last update date Jun 05, 2024
Contact name Philippe Collas
Organization name University of Oslo
Department Institute of Basic Medical Sciences
Street address PO Box 1112 Blindern
City Oslo
ZIP/Postal code 0317
Country Norway
 
This SubSeries is part of SuperSeries:
GSE256262 Alternative isoform expression of key thermogenic genes in human beige adipocytes
Relations
Reanalysis of GSM1443809
Reanalysis of GSM1443810
Reanalysis of GSM1443811
Reanalysis of GSM1443812
Reanalysis of GSM1537677
Reanalysis of GSM1537678
Reanalysis of GSM1537679
Reanalysis of GSM1537680
Reanalysis of GSM1443817
Reanalysis of GSM1443818

reanalyzed_samples_from_GSE59703 header descriptions
GEO Sample
GEO title
cell line
cell type
antibody
data processing step
data processing step
data processing step
data processing step
assembly
processed data files format and content
processed data files format and content

Data table
GEO Sample GEO title cell line cell type antibody data processing step data processing step data processing step data processing step assembly processed data files format and content processed data files format and content
GSM1443809 PPARgamma hMADS white rep1, day 19 hMADS-3 human adipose-derived stem cells anti-PPAR? (H-100, sc-7196; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1443810 PPARgamma hMADS brite rep1, day 19 hMADS-3 human adipose-derived stem cells anti-PPAR? (H-100, sc-7196; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1443811 PPARgamma hMADS white rep2, day 19 hMADS-3 human adipose-derived stem cells anti-PPAR? (H-100, sc-7196; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1443812 PPARgamma hMADS brite rep2, day 19 hMADS-3 human adipose-derived stem cells anti-PPAR? (H-100, sc-7196; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1537677 MED1 hMADS white rep1, day 19 hMADS-3 human adipose-derived stem cells MED1 (M-255; sc-8998; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1537678 MED1 hMADS brite rep1, day 19 hMADS-3 human adipose-derived stem cells MED1 (M-255; sc-8998; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1537679 MED1 hMADS white rep2, day 19 hMADS-3 human adipose-derived stem cells MED1 (M-255; sc-8998; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1537680 MED1 hMADS brite rep2, day 19 hMADS-3 human adipose-derived stem cells MED1 (M-255; sc-8998; Santa Cruz) PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1443817 Input hMADS white rep1, day 19 hMADS-3 human adipose-derived stem cells None PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38
GSM1443818 Input hMADS brite rep1, day 19 hMADS-3 human adipose-derived stem cells None PPARgamma and MED1 ChIP-Seq reads for white and brite adipocytes were downloaded from Gene Expression Omnibus (GEO) accession GSE59703 (Loft et al. 2015). Reads from replicates were merged and aligned to hg38 using Bowtie2 v2.4.5. Peaks were detected using MACS3 v3.0.0b3. ChIP/Input ratio tracks were generated using bamCompare in deepTools v3.5.3. hg38 ChIP/Input ratio tracks for merged replicates in hg38 Narrow and broad peaks for merged replicates in hg38

Total number of rows: 10


Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE256261_GSM1443809_and_GSM1443811_PPARgamma_hMADS_white_peaks.narrowPeak.gz 1.7 Mb (ftp)(http) NARROWPEAK
GSE256261_GSM1443809_and_GSM1443811_PPARgamma_white_hMADS_D19_ratio.bw 492.7 Mb (ftp)(http) BW
GSE256261_GSM1443810_and_GSM1443812_PPARgamma_brite_hMADS_D19_ratio.bw 506.7 Mb (ftp)(http) BW
GSE256261_GSM1443810_and_GSM1443812_PPARgamma_hMADS_brite_peaks.narrowPeak.gz 2.0 Mb (ftp)(http) NARROWPEAK
GSE256261_GSM1537677_and_GSM1537679_MED1_white_hMADS_D19_ratio.bw 654.1 Mb (ftp)(http) BW
GSE256261_GSM1537677_and_GSM1537679_MED1_white_peaks.broadPeak.gz 1.2 Mb (ftp)(http) BROADPEAK
GSE256261_GSM1537678_and_GSM1537680_MED1_brite_hMADS_D19_ratio.bw 685.4 Mb (ftp)(http) BW
GSE256261_GSM1537678_and_GSM1537680_MED1_brite_peaks.broadPeak.gz 1.4 Mb (ftp)(http) BROADPEAK

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap