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| Status |
Public on Mar 04, 2024 |
| Title |
Effects of PCB 52 Exposure Via Sub-Acute Nose-Only Inhalation on Gene Expression in Fat Depots of Adolescent Rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Polychlorinated biphenyls (PCBs) are persistent environmental toxicants that pose a variety of health risks in humans. PCB 52 has been found in the indoor air of schools where adolescent students and staff are being actively exposed. This study examined the three different fat depots, namely, brown adipose tissue (BAT), subcutaneous adipose tissue (SAT), and visceral adipose tissue (VAT), of adolescent Sprague-Dawley rats after sub-acute exposure to PCB 52 via nose-only inhalation. Rats were exposed for 4 hours daily for 28 consecutive days. RNA extracted from both male and female fat depots were submitted for RNA sequencing to compare the effects of PCB 52 on the expression of genes in the fat depots.
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| Overall design |
To determine the gene expression alterations following PCB 52 inhalation exposure in adolescent rats, samples from male and female fat depots (BAT, SAT, and VAT) were processed for RNA sequencing. (N = 6 per sex; Samples were excluded if RNA RINs were below 8).
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| Contributor(s) |
Gourronc FA, Bullert A, Wang H, Helm-Kwasny BK, Chimenti M, Adamcakova-Dodd A, Jing X, Ankrum JA, Thorne PS, Lehmler H, Klingelhutz AJ |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P42 ES013661 |
Airborne PCBs: Sources, Exposures, Toxicities, Remediation |
THE UNIVERSITY OF IOWA |
Keri C Hornbuckle |
| P30 ES005605 |
Environmental Health Sciences Research Center |
THE UNIVERSITY OF IOWA |
Peter S Thorne |
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| Submission date |
Feb 27, 2024 |
| Last update date |
Mar 04, 2024 |
| Contact name |
Aloysius John Klingelhutz |
| E-mail(s) |
al-klingelhutz@uiowa.edu
|
| Organization name |
University of Iowa
|
| Department |
Microbiology and Immunology
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| Lab |
Klingelhutz Lab
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| Street address |
3-612 BSB, 51 Newton Road
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (70)
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| Relations |
| BioProject |
PRJNA1081373 |
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