GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE25938 Query DataSets for GSE25938
Status Public on Dec 10, 2010
Title Developmental stages after fertilization in Salmo salar
Organism Salmo salar
Experiment type Expression profiling by array
Summary Atlantic salmon (Salmo salar L.) is an environmentally and economically important organism and its gene content is reasonably well characterized. From a transcriptional standpoint, it is important to characterize the normal changes in gene expression over the course of early development, from fertilization through to the parr stage.S. salar samples were taken at 17 time points from 2 to 89 days post fertilization. Total RNA was extracted and cRNA was synthesized and hybridized to a new 44K oligo salmonid microarray platform. Quantified results were subjected to preliminary data analysis and submitted to NCBI’s Gene Expression Omnibus. Throughout the entire period of development, several thousand genes were found to be differentially regulated. This work represents the trancriptional characterization of a very large geneset that will be extremely valuable in further examination of the transcriptional changes in Atlantic salmon during the first few months of development. The expression profiles can help to annotate salmon genes in addition to being used as references against any number of experimental variables that developing salmonids might be subjected to.
Overall design 17 time points (2 - 89 dpf) with three samples for each time point
Equimolar amounts from all three individuals in each time point were pooled. Then reference pool was created by including equal quantities cRNA from each time point, except for the first time point which had less material included.
Contributor(s) Jantzen SG, Sanderson DS, von Schalburg KR, Yasuike M
Citation(s) 21447175
Submission date Dec 08, 2010
Last update date Feb 14, 2019
Contact name Ben F Koop
Phone (250) 472-4067
Organization name The University of Victoria
Department Biology
Lab Centre for Biomedical Research
Street address PO Box 3020 STN CSC
City Victoria
State/province BC
ZIP/Postal code V8W 3N5
Country Canada
Platforms (1)
GPL11299 Agilent-025055 Koop Lab Salmonid 44k
Samples (51)
GSM637444 S. salar_47_dpf_rep1
GSM637445 S. salar_65_dpf_rep1
GSM637446 S. salar_35_dpf_rep1
BioProject PRJNA135691

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25938_RAW.tar 16.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap