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| Status |
Public on Sep 01, 2011 |
| Title |
Expression profiling of the Staphylococcus aureus GraSR regulon |
| Platform organism |
Staphylococcus aureus subsp. aureus NCTC 8325 |
| Sample organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
The GraS/GraR two-component system has been shown to control cationic antimicrobial peptide (CAMP) resistance in the major human pathogen Staphylococcus aureus. We identified a highly conserved ten base pair palindromic sequence (5’ ACAAATTTGT 3’) located upstream from GraR-regulated genes (mprF and the dltA and vraFG operons), which we show to be essential for transcriptional regulation by GraR and induction in response to colistin, a bacterial CAMP, suggesting it is the likely GraR binding site. Genome-based predictions and transcriptome analysis revealed several novel GraR target genes.
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| Overall design |
Global expression changes between the wild type strain HG001 (a rsbU+ variant of strain NCTC 8325) and a DgraSR mutant, grown to mid-exponential phase in TSB with 50 µg/ml colistin, were examined. Hybridizations were performed in triplicate using RNA isolated from independent cultures.
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| Contributor(s) |
Falord M, Mäder U, Msadek T |
| Citation(s) |
21765893 |
| |
| Submission date |
Dec 10, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Ulrike Mäder |
| E-mail(s) |
ulrike.maeder@uni-greifswald.de
|
| Organization name |
University Medicine Greifswald
|
| Department |
Functional Genomics
|
| Street address |
F.-L.-Jahn-Str. 15A
|
| City |
Greifswald |
| ZIP/Postal code |
D-17489 |
| Country |
Germany |
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| Platforms (1) |
| GPL11308 |
BaSysBio Staphylococcus aureus T1 385K array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA135479 |
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