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| Status |
Public on Apr 01, 2024 |
| Title |
Positional information modulates transient regeneration-activated cell states during vertebrate appendage regeneration. |
| Organism |
Nothobranchius furzeri |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Injury is a common occurrence in the life of most if not all organisms. Because injury and the extent of damage inflicted are not predictable events, pre-existing tissues must somehow determine how much tissue needs to be restored. It has been known for over a century that amputation position along a proximal-distal (P/D) axis determines the regeneration speed of amputated limbs in regeneration-competent animals1. Yet, it is not clear how an injured limb conveys positional information during regeneration to faithfully restore pre-existing morphologies and functions. Here, we profiled millimetric caudal fins regenerating proximal and distal amputations in the African killifish (Nothobranchius furzeri). We report position-specific, differential modulation of the spatial distribution, duration, and magnitude of proliferation, and the possibility to predict blastema sizes. Regenerating fins profiled by single cell RNA sequencing identified a Transient Regeneration-Activated Cell State (TRACS) enriched in extracellular matrix (ECM) components and modifiers that is proportionally amplified to match a given amputation position. We located this transient cell state to the basal epidermis by gene expression and confocal microscopy, and propose a role for these cells in transducing positional information to the regeneration blastema. Understanding the temporal deployment of regenerative cell states may hold the key to set up a successful regeneration event in organisms with limited regenerative abilities such as humans.
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| Overall design |
Regenerating or homeostasis cells from caudal fins or dorsal fins were isolated by FACS according to live/nucleated signal and analyzed using scRNA-seq
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| Contributor(s) |
Ortega Granillo A, Zamora D, Schnittker RR, Scott AR, Russell J, Brewster CE, Ross EJ, Acheampong D, Ferro K, Morrison JA, Rubinstein BY, Perera AG, Wang W, Sánchez Alvarado A |
| Citation(s) |
39286507 |
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| Submission date |
Feb 29, 2024 |
| Last update date |
Sep 19, 2024 |
| Contact name |
Alejandro Sanchez Alvarado |
| E-mail(s) |
asa@stowers.org
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| Organization name |
Stowers Institute for Medical Research
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| Lab |
Sanchez Alvarado Lab
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| Street address |
1000 East 50th Street
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| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
61410 |
| Country |
USA |
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| Platforms (1) |
| GPL32658 |
Illumina NovaSeq 6000 (Nothobranchius furzeri) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA1082368 |
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