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| Status |
Public on Mar 07, 2024 |
| Title |
Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments |
| Organism |
Chlamydomonas reinhardtii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Maintenance of water homeostasis is a fundamental cellular process required by all living organisms. Here, we use the single-celled green alga Chlamydomonas reinhardtii to establish a foundational understanding of osmotic-stress signaling pathways through transcriptomics, phosphoproteomics, and functional genomics approaches. Comparison of pathways identified through these analyses with yeast and Arabidopsis allowed us to infer their evolutionary conservation and divergence across these lineages. 76 genes, acting across diverse cellular compartments, were found to be important for osmotic-stress tolerance in Chlamydomonas through their functions in cytoskeletal organization, potassium transport, vesicle trafficking, mitogen-activated protein kinase and chloroplast signaling. We show that homologs for five of these genes have conserved functions in stress tolerance in Arabidopsis and reveal a novel PROFILIN-dependent stage of acclimation affecting the actin cytoskeleton that ensures tissue integrity upon osmotic stress. This study highlights the conservation of the stress response in algae and land plants and establishes Chlamydomonas as a unicellular plant model system to dissect the osmotic stress signaling pathway.
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| Overall design |
Investigate the gene expression upon osmotic treatment using sodium chloride and mannitol of Chlamydomonas reihardtii (CC-4533)
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| Web link |
https://www.biorxiv.org/content/10.1101/2021.07.19.453009
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| Contributor(s) |
Vilarrasa Blasi J, Dinneny JR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 04, 2024 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Josep Vilarrasa Blasi |
| E-mail(s) |
pituvilarnadal@gmail.com
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| Phone |
6506563386
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| Organization name |
Aetna
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| Street address |
787 corto street
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| City |
Mountain View |
| State/province |
CA |
| ZIP/Postal code |
94043 |
| Country |
USA |
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| Platforms (1) |
| GPL34271 |
NextSeq 2000 (Chlamydomonas reinhardtii) |
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| Samples (45)
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| GSM8124912 |
Chlamydomonas, Control, 0 hours, rep1 |
| GSM8124913 |
Chlamydomonas, NaCl, 4 hours, rep2 |
| GSM8124914 |
Chlamydomonas, Mannitol, 6 hours, rep1 |
| GSM8124915 |
Chlamydomonas, Control, 0 hours, rep2 |
| GSM8124916 |
Chlamydomonas, Mannitol, 4 hours, rep1 |
| GSM8124917 |
Chlamydomonas, Mannitol, 15 minutes, rep2 |
| GSM8124918 |
Chlamydomonas, NaCl, 30 minutes, rep1 |
| GSM8124919 |
Chlamydomonas, NaCl, 30 minutes, rep2 |
| GSM8124920 |
Chlamydomonas, NaCl, 15 minutes, rep1 |
| GSM8124921 |
Chlamydomonas, Control, 0 hours, rep3 |
| GSM8124922 |
Chlamydomonas, Mannitol, 60 minutes, rep1 |
| GSM8124923 |
Chlamydomonas, Mannitol, 6 hours, rep2 |
| GSM8124924 |
Chlamydomonas, NaCl, 6 hours, rep1 |
| GSM8124925 |
Chlamydomonas, Mannitol, 30 minutes, rep1 |
| GSM8124926 |
Chlamydomonas, Mannitol, 4 hours, rep2 |
| GSM8124927 |
Chlamydomonas, Control 24 hours, rep2 |
| GSM8124928 |
Chlamydomonas, Mannitol, 30 minutes, rep2 |
| GSM8124929 |
Chlamydomonas, Mannitol, 60 minutes, rep2 |
| GSM8124930 |
Chlamydomonas, NaCl , 24 hours, rep1 |
| GSM8124931 |
Chlamydomonas, Control, 0 hours, rep4 |
| GSM8124932 |
Chlamydomonas, NaCl , 24 hours, rep2 |
| GSM8124933 |
Chlamydomonas, Mannitol, 24 hours, rep1 |
| GSM8124934 |
Chlamydomonas, NaCl, 60 min, rep2 |
| GSM8124935 |
Chlamydomonas, NaCl, 15 minutes, rep2 |
| GSM8124936 |
Chlamydomonas, NaCl, 6 hours, rep2 |
| GSM8124937 |
Chlamydomonas, Mannitol, 24 hours, rep2 |
| GSM8124938 |
Chlamydomonas, Control 24 hours, rep3 |
| GSM8124939 |
Chlamydomonas, Control 24 hours, rep4 |
| GSM8124940 |
Chlamydomonas, Control, 0 hours, rep5 |
| GSM8124941 |
Chlamydomonas, NaCl, 15 minutes, rep3 |
| GSM8124942 |
Chlamydomonas, Mannitol, 15 minutes, rep3 |
| GSM8124943 |
Chlamydomonas, Mannitol, 30 minutes, rep3 |
| GSM8124944 |
Chlamydomonas, NaCl, 30 minutes, rep3 |
| GSM8124945 |
Chlamydomonas, NaCl, 60 min, rep3 |
| GSM8124946 |
Chlamydomonas, Mannitol, 4 hours, rep3 |
| GSM8124947 |
Chlamydomonas, Mannitol, 60 minutes, rep3 |
| GSM8124948 |
Chlamydomonas, NaCl, 4 hours, rep3 |
| GSM8124949 |
Chlamydomonas, Mannitol, 6 hours, rep3 |
| GSM8124950 |
Chlamydomonas, NaCl, 6 hours, rep3 |
| GSM8124951 |
Chlamydomonas, Mannitol, 24 hours, rep3 |
| GSM8124952 |
Chlamydomonas, NaCl , 24 hours, rep3 |
| GSM8124953 |
Chlamydomonas, Control 24 hours, rep5 |
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| Relations |
| BioProject |
PRJNA1083559 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE260814_Geo_TPM_RNaseq_Chlamy_osmotic.txt.gz |
3.7 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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