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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 27, 2024 |
| Title |
Exosomal miRNA profiling in Bcl-w overexpressed U251cells |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Exosomes are cell membrane-derived endoplasmic reticulum measuring 30 to 120 nm, which are secreted from various cells including cancer cells, and consist of lipids, proteins, mRNAs, and microRNAs (miRNAs). Tumor-derived exosomes are involved in tumor progression by delivering various factors to neighboring cells and promoting intercellular communication. MiRNA is a non-coding RNA consisting of 20-24 nucleotides that binds to the 3'UTR region of the target gene with a complementary nucleotide sequence during gene expression to degrade target mRNA or inhibit translation into target protein. In our study, exosomal miRNA profiling was utilized as a crucial technique to analyze the mechanisms of intercellular communication in tumor malignancy
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| Overall design |
We isolated exosomes from the conditioned medium of U251 cells overexpressing vector and Bcl-w. RNA was extracted from isolated exosomes and performed on a miRNA microarray.
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| Contributor(s) |
Bae IH, Choi JY, Seok HJ, Lee DH |
| Citation(s) |
38521953 |
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| Submission date |
Mar 05, 2024 |
| Last update date |
Mar 28, 2024 |
| Contact name |
IN HWA BAE |
| E-mail(s) |
ihbae@kirams.re.kr
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| Organization name |
Korea Institute of Radiological & Medical Sciences
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| Street address |
75 Nowon-ro, Nowon-gu
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| City |
Seoul |
| State/province |
--- Select One --- |
| ZIP/Postal code |
01812 |
| Country |
South Korea |
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| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1083993 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE260909_RAW.tar |
1.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
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