|
| Status |
Public on May 20, 2024 |
| Title |
Acetylation is not mere a consequence of transcription: histones are acetylated without ongoing transcription [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In all eukaryotes, acetylation of histones lysine residues correlates with transcription activation. Whether histone acetylation is a cause or consequence of transcription is debated. One model suggests that transcription promotes the recruitment and/or activation of acetyltransferases and histone acetylation occurs as a consequence of ongoing transcription. However, the extent to which transcription shapes the global acetylation landscapes is not known. Here we show that global protein acetylation remains virtually unaltered after acute transcription inhibition. Transcription inhibition ablates the co-transcriptionally occurring ubiquitylation of H2BK120 but does not reduce histone acetylation. The combined inhibition of transcription and CBP/p300 further demonstrates that acetyltransferases remain active and continue to acetylate histones independently of transcription. Together, these results show that histone acetylation is not a mere consequence of transcription; acetyltransferase recruitment and activation is uncoupled from the act of transcription and histone and non-histone protein acetylation are sustained in the absence of ongoing transcription.
|
| |
|
| Overall design |
H3K27ac ChIP-seq experiments in ESC with or without actinomycin D or NVP-2 treatment. EU-seq experiments in ESC with triptolide treatment.
|
| |
|
| Contributor(s) |
Narita T, Hitoshi A, Elina M, Choudhary C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 05, 2024 |
| Last update date |
May 20, 2024 |
| Contact name |
Chunarum Choudhary |
| E-mail(s) |
chuna.choudhary@cpr.ku.dk
|
| Organization name |
University of Copenhagen
|
| Department |
Novo Nordisk Foundation Center for Protein Research
|
| Lab |
Choudhary Group
|
| Street address |
Blegdamsvej 3B
|
| City |
Copenhagen N |
| State/province |
--- Select a state --- |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
|
| Samples (6)
|
| GSM8128093 |
H3K27ac ChIP-seq in ESC rep1 |
| GSM8128094 |
H3K27ac ChIP-seq in ESC rep2 |
| GSM8128095 |
H3K27ac ChIP-seq in ESC after NVP-2 treatment for 120min treatment rep1 |
| GSM8128096 |
H3K27ac ChIP-seq in ESC after NVP-2 treatment for 120min treatment rep2 |
| GSM8128097 |
H3K27ac ChIP-seq in ESC after actinomycin D treatment for 120min treatment rep1 |
| GSM8128098 |
H3K27ac ChIP-seq in ESC after actinomycin D treatment for 120min treatment rep2 |
|
| This SubSeries is part of SuperSeries: |
| GSE260969 |
Acetylation is not mere a consequence of transcription: histones are acetylated without ongoing transcription |
|
| Relations |
| BioProject |
PRJNA1084008 |
Less...
More...