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| Status |
Public on Jun 05, 2024 |
| Title |
Spatial Transcriptomics Unravels Palmitoylation and Zonation-Dependent Gene Regulation by AEG-1 in Mouse Liver |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Obesity-induced metabolic dysfunction-associated steatohepatitis (MASH) leads to hepatocellular carcinoma (HCC). Astrocyte-elevated gene-1/Metadherin (AEG-1/MTDH) plays a key role in promoting MASH and HCC. AEG-1 is palmitoylated at residue cysteine 75 (Cys75) and a knock-in mouse representing mutated Cys75 to serine (AEG-1-C75S) showed activation of MASH- and HCC-promoting gene signature when compared to wild-type littermates (AEG-1-WT). The liver consists of 3 zones, periportal, midlobular and pericentral, and zone-specific dysregulated gene expression impairs metabolic homeostasis in the liver, contributing to MASH and HCC. Here, to elucidate how palmitoylation influences AEG-1-mediated gene regulation in regards to hepatic zonation, we performed spatial transcriptomics (ST) in the livers of AEG-1-WT and AEG-1-C75S littermates.
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| Overall design |
Liver sections of female AEG-1-WT and AEG-1-C75S littermates were subjected to spatial transcriptomics.
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| Contributor(s) |
Saverino A, Qu X, Mendoza RG, Raha S, Manna D, Ermi AG, Subler MA, Windle JJ, Liu J, Sarkar D |
| Citation(s) |
38677511 |
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| Submission date |
Mar 07, 2024 |
| Last update date |
Jun 06, 2024 |
| Contact name |
Wright Center for Clinical and Translational Research Bioinformatics Core |
| E-mail(s) |
cctrbioinfo@vcu.edu
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| Phone |
804-828-1621
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| Organization name |
Virginia Commonwealth University
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| Street address |
203 E Cary St
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| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23298 |
| Country |
USA |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1085111 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE261057_RAW.tar |
490.5 Mb |
(http)(custom) |
TAR (of MTX, TIFF, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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