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| Status |
Public on Jul 23, 2024 |
| Title |
Gene expression profile at single cell level of non-parenchymal cells from Fibrosis and Tumor (FAT) Metabolic Dysfunction-Associated Steatohepatitis (MASH) model vs healthy liver at 12- and 24-week timepoints. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
MASH is a subtype of metabolic dysfunction-associated steatotic liver disease, characterized by inflammation and fibrosis. Non-parenchymal cells in this dataset include mostly immune cells, as well as some other cell types like endothelial cells, cholangiocytes, stellate cells, etc.
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| Overall design |
Single cell suspensions from MASH vs healthy murine livers were made using enzymatic digestion with Dnase I and Collagenase IV, as well as mechanical digestion, followed by filtering. Non-parenchymal cells were enriched by using percoll gradients (70% and 25%). Most hepatocytes are removed with this protocol, and red blood cells are lysed. Samples were submitted for scRNAseq, and were analyzed for uPAR expression in this study.
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| Contributor(s) |
Yashaswini CN, Friedman SL |
| Citation(s) |
38508241 |
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| Submission date |
Mar 07, 2024 |
| Last update date |
Jul 24, 2024 |
| Contact name |
Chittampalli Narayanan Yashaswini |
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Liver
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| Lab |
Friedman Lab
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| Street address |
1425 Madison Avenue, 11-76
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1085495 |
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