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| Status |
Public on May 11, 2024 |
| Title |
Cardiac Outflow tract septation defects in a DiGeorge syndrome model respond to Minoxidil treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We found that Tbx1 suppresses a subset of ECM-related genes in a cell differentiation model. Treatment with a Lysyl hydroxylase inhibitor drug in vivo during pregnancy reduced the expressivity of cardiac defects in Tbx1 mutant embryos.
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| Overall design |
E14-Tg2a cells (parental line and Tbx1-/- line 4D) were dissociated with Trypsin-EDTA and cultured at a density of 100,000 cells/ml in serum-free differentiation media. We followed the protocol described by Andersen et al Nat Commun 9, 3140 (2018) and harvested cells at day 6 of differentiation. We processed for QuantSeq 4 biological replicates of the parental line and 4 of the 4D line.
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| Contributor(s) |
Ferrentino R, Aurigemma I, Lanzetta O, Poondi Krishnan V, Angelini C, Illingworth E, Baldini A |
| Citation(s) |
38749189 |
| |
| Submission date |
Mar 08, 2024 |
| Last update date |
Aug 12, 2024 |
| Contact name |
Antonio Baldini |
| E-mail(s) |
antonio.baldini@unina.it
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| Organization name |
University of Naples Federico II
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| Department |
Department of Molecular Medicine and Medical Biotechnology
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| Street address |
Via Sergio Pansini 5
|
| City |
Naples |
| State/province |
Naples |
| ZIP/Postal code |
80131 |
| Country |
Italy |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1085754 |
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