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Series GSE261285 Query DataSets for GSE261285
Status Public on Jun 30, 2024
Title small RNA-sequencing analysis of db/db mice exudate from subcutaneously implanted sponges
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Dried polyvinyl alcohol (PVA) sponges were used for subcutaneous implantation in the mice dorsum, and were harvested on days 3, 5, 10, and 16 post-implantation.
 
Overall design Male 16-week old db/db mice (stock # 000642) were obtained from Jackson Laboratories and were acclimated to the animal facility for at least one week before surgery. A vertical incision of 2 cm was created on depilated and disinfected dorsum and a pocket was formed by separating the skin from the muscle to insert 4-6 PVA sponges. Once insertions were completed, the incisions were sutured and dermabond was applied. On collection days, PVA sponges containing wound fluids were collected in empty 5 ml syringes, placed in 15 ml falcon tubes and centrifuged for fluid collection at 3,000 rpm for 10 minutes at 4 °C.
 
Contributor(s) Kalavros N, Theocharidis G
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 11, 2024
Last update date Jul 01, 2024
Contact name Nikolaos Kalavros
E-mail(s) nkalavro@bidmc.harvard.edu
Phone 8573526152
Organization name Beth Israel Deaconess Medical Center
Department Pathology
Lab Spatial Technologies Unit
Street address 330 Brookline Avenue
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (20)
GSM8139456 Sample21
GSM8139457 Sample22
GSM8139458 Sample23
Relations
BioProject PRJNA1086392

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE261285_RAW.tar 7.0 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA

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