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Status |
Public on Jun 30, 2024 |
Title |
small RNA-sequencing analysis of db/db mice exudate from subcutaneously implanted sponges |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Dried polyvinyl alcohol (PVA) sponges were used for subcutaneous implantation in the mice dorsum, and were harvested on days 3, 5, 10, and 16 post-implantation.
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Overall design |
Male 16-week old db/db mice (stock # 000642) were obtained from Jackson Laboratories and were acclimated to the animal facility for at least one week before surgery. A vertical incision of 2 cm was created on depilated and disinfected dorsum and a pocket was formed by separating the skin from the muscle to insert 4-6 PVA sponges. Once insertions were completed, the incisions were sutured and dermabond was applied. On collection days, PVA sponges containing wound fluids were collected in empty 5 ml syringes, placed in 15 ml falcon tubes and centrifuged for fluid collection at 3,000 rpm for 10 minutes at 4 °C.
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Contributor(s) |
Kalavros N, Theocharidis G |
Citation missing |
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Submission date |
Mar 11, 2024 |
Last update date |
Jul 01, 2024 |
Contact name |
Nikolaos Kalavros |
E-mail(s) |
nkalavro@bidmc.harvard.edu
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Phone |
8573526152
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Organization name |
Beth Israel Deaconess Medical Center
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Department |
Pathology
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Lab |
Spatial Technologies Unit
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Street address |
330 Brookline Avenue
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City |
Boston |
State/province |
Massachusetts |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (20)
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Relations |
BioProject |
PRJNA1086392 |