|
| Status |
Public on Jun 20, 2024 |
| Title |
Single-cell RNAseq of Neurog1-Neurog2-DsRed transduced DRG cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cultured adult mouse dorsal root ganglia (DRG) cells exhibit glial sensory progenitor properties in vitro. Therefore, they might be a good starter cell for reprogramming into sensory neurons. Here, we infected them with the retroviral vector Neurog2-Neurog1-DsRed to induce sensory neuron development and analyzed by scRNAseq at 14 days post infection whether infected cells show properties of sensory neurons such as nociceptors. After 10x Genomics, data analysis of 4,549 individual cells indicated the generation of neurons, but at an immature cell state.
|
| |
|
| Overall design |
Cells from dorsal root ganglia culture of adult mice were infected with Neurog2-Neurog1-DsRed, DsRed-positive cells were isolated with Fluorescence-activated cell sorting (FACS) at 14 days post infection and analyzed using scRNAseq.
|
| Web link |
https://www.biorxiv.org/content/10.1101/2024.04.09.588701v1.full.pdf
|
| |
|
| Contributor(s) |
Sodmann A, Blum R, Imdahl F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 13, 2024 |
| Last update date |
Jun 21, 2024 |
| Contact name |
Robert Blum |
| E-mail(s) |
Blum_R@UKW.de
|
| Phone |
+49 931 201 23672
|
| Organization name |
University Hospital Würzburg
|
| Department |
Department of Neurology
|
| Lab |
AG Blum
|
| Street address |
Josef-Schneider-Str. 11
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platforms (1) |
|
| Samples (1) |
| GSM8146176 |
mouse DRG cell culture, 14 days post infection with Neurog2-Neurog1-DsRed, rep1 |
|
| Relations |
| BioProject |
PRJNA1087386 |
