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Series GSE262579 Query DataSets for GSE262579
Status Public on May 08, 2024
Title Bacteria conjugate ubiquitin-like proteins to interfere with phage assembly
Organisms Escherichia coli; Caulobacter sp. Root343
Experiment type Expression profiling by high throughput sequencing
Summary Multiple immune pathways in humans conjugate ubiquitin-like proteins to virus and host molecules as a means of antiviral defense. Here we studied an anti-phage defense system in bacteria, comprising a ubiquitin-like protein, ubiquitin-conjugating enzymes E1 and E2, and a deubiquitinase. We show that during phage infection, this system specifically conjugates the ubiquitin-like protein to the phage central tail fiber, a protein at the tip of the tail that is essential for tail assembly as well as for recognition of the target host receptor. Following infection, cells encoding this defense system release a mixture of partially assembled, tailless phage particles, and fully assembled phages in which the central tail fiber is obstructed by the covalently attached ubiquitin-like protein. These phages exhibit severely impaired infectivity, explaining how the defense system protects the bacterial population from the spread of phage infection. Our findings demonstrate that conjugation of ubiquitin-like proteins is an antiviral strategy conserved across the tree of life.
Overall design Total RNA-seq of Caulobacter sp. Root343 at different growth phases, as well as E. coli expressing the Bil system from a plasmid
Contributor(s) Hör J, Wolf SG, Sorek R
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Submission date Mar 27, 2024
Last update date May 08, 2024
Contact name Jens Hör
Organization name Weizmann Institute for Science
Lab Rotem Sorek
Street address 234 Herzl Street
City Rehovot
ZIP/Postal code 7610001
Country Israel
Platforms (2)
GPL32081 NextSeq 2000 (Escherichia coli)
GPL34341 NextSeq 2000 (Caulobacter sp. Root343)
Samples (12)
GSM8172000 Caulobacter_mid_log_R1
GSM8172001 Caulobacter_mid_log_R2
GSM8172002 Caulobacter_mid_log_R3
BioProject PRJNA1092583

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Supplementary file Size Download File type/resource
GSE262579_Caulobacter_TPM_counts.xlsx 1.2 Mb (ftp)(http) XLSX
GSE262579_E-coli_TPM_counts.xlsx 394.9 Kb (ftp)(http) XLSX
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