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| Status |
Public on Apr 03, 2024 |
| Title |
Changes in the cellular makeup of motor patterning circuits drive courtship song evolution in Drosophila |
| Organisms |
Drosophila melanogaster; Drosophila yakuba |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
How evolutionary changes in genes and neurons encode species variation in complex motor behaviors are largely unknown. Here, we develop genetic tools that permit a neural circuit comparison between the model speciesDrosophila melanogasterand the closely-related speciesD. yakuba, who has undergone a lineage-specific loss of sine song, one of the two major types of male courtship song inDrosophila. Neuroanatomical comparison of song patterning neurons called TN1 across the phylogeny demonstrates a link between the loss of sine song and a reduction both in the number of TN1 neurons and the neurites serving the sine circuit connectivity. Optogenetic activation confirms that TN1 neurons inD. yakubahave lost the ability to drive sine song, while maintaining the ability to drive the singing wing posture. Single-cell transcriptomic comparison shows thatD. yakubaspecifically lacks a cell type corresponding to TN1A neurons, the TN1 subtype that is essential for sine song. Genetic and developmental manipulation reveals a functional divergence of the sex determination genedoublesexinD. yakubato reduce TN1 number by promoting apoptosis. Our work illustrates the contribution of motor patterning circuits and cell type changes in behavioral evolution, and uncovers the evolutionary lability of sex determination genes to reconfigure the cellular makeup of neural circuits.
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| Overall design |
Doublesex expressing neurons from male flies were isolated by FACS based on the expression of tdTomato and analyzed by scRNAseq
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| Contributor(s) |
Ye D, Walsh J, Junker I, Ding Y |
| Citation missing |
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| Submission date |
Mar 28, 2024 |
| Last update date |
Apr 03, 2024 |
| Contact name |
Justin Walsh |
| E-mail(s) |
juswalsh@sas.upenn.edu
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| Phone |
2676149624
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| Organization name |
University of Pennsylvania
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| Street address |
3740 Hamilton Walk
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (2) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
| GPL33170 |
Illumina NovaSeq 6000 (Drosophila yakuba) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1093075 |
