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| Status |
Public on Mar 31, 2024 |
| Title |
Comparative MicroRNA Sequencing Analysis in Late L1 Larvae of C. elegans: Wild Type N2 Versus myrf-1(ju1121) Mutants |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Our research revealed that the transmembrane transcription factors MYRF-1 and MYRF-2 are crucial for activating the microRNA lin-4 at the end of the first larval stage in C. elegans. To determine whether MYRF regulates other microRNA genes during this period, we conducted a microRNA sequencing analysis to compare microRNA expression in late L1 larvae of wild type and myrf-1(ju1121) mutants. The myrf-1(ju1121) mutation causes a loss of function in both MYRF-1 and MYRF-2. Our analysis identified a subset of microRNAs that were differentially expressed between the wild type and the myrf-1(ju1121) mutants.
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| Overall design |
Late L1 larvae from both wild type and myrf-1(ju1121) mutants, each harboring the transgene marker Phlh-8-GFP(ayIs6), were gathered. RNA was subsequently extracted from these samples and subjected to microRNA sequencing and analysis.
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| Web link |
https://doi.org/10.7554/eLife.89903.1.sa2
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| Contributor(s) |
Xu Z, Wang Z, Wang L, Qi YB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 28, 2024 |
| Last update date |
Mar 31, 2024 |
| Contact name |
LIFANG WANG |
| E-mail(s) |
lifangwang06@gmail.com
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| Organization name |
SHANGHAITECH
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| Street address |
393 Middle Huaxia Road
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| City |
Shanghai |
| ZIP/Postal code |
201210 |
| Country |
China |
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| Platforms (1) |
| GPL26672 |
Illumina NovaSeq 6000 (Caenorhabditis elegans) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1093146 |
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