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| Status |
Public on Jun 25, 2024 |
| Title |
The Rubicon-WIPI axis regulates exosome biogenesis during aging |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Cells release intraluminal vesicles (ILVs) in multivesicular bodies as exosomes to communicate with other cells. Although recent studies suggest an intimate link between exosome biogenesis and autophagy, the detailed mechanism is not fully understood. Here we employed comprehensive RNAi screening for autophagy-related factors and discovered that Rubicon, a negative regulator of autophagy, is essential for exosome release. Rubicon recruits WIPI2d to endosomes to promote exosome biogenesis. Interactome analysis of WIPI2d identified the ESCRT components that are required for ILV formation. Notably, we found that Rubicon is required for an age-dependent increase of exosome release in mice. In addition, small RNA sequencing of serum exosomes revealed that Rubicon determines the fate of exosomal microRNAs associated with aging and longevity pathways. Taken together, our current results suggest that the Rubicon-WIPI axis functions as a key regulator of exosome biogenesis and is responsible for the age-dependent changes in exosome quantity and quality.
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| Overall design |
Exosomes isolated from young and aged mice with or without Rubicon knockout
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| Contributor(s) |
Yanagawa K, Yoshimori T, Edahiro R |
| Citation missing |
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| |
| Submission date |
Mar 29, 2024 |
| Last update date |
Jun 25, 2024 |
| Contact name |
Ryuya Edahiro |
| E-mail(s) |
ryuya.edahiro@gmail.com
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| Organization name |
Osaka University
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| Street address |
Yamadaoka2-2
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| City |
Suita |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1093398 |
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