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Series GSE263777 Query DataSets for GSE263777
Status Public on Apr 14, 2024
Title Two distinct Trypanosoma eIF4F complexes co-exist, bind different mRNAs and are regulated during nutritional stress
Organism Trypanosoma cruzi
Experiment type Expression profiling by high throughput sequencing
Other
Summary Many eIF4F subunits and PABP paralogues are found in trypanosomes: six eIF4E, five eIF4G, one eIF4A and two PABPs. They are expressed simultaneously and assemble into different complexes, contrasting the situation in metazoans that use distinct complexes in different cell types or developmental stages. Each eIF4F complex has its own proteins, mRNAs and, consequently, a distinct function. We set out to study the function and regulation of the two major eIF4F complexes of Trypanosoma cruzi. We identified the associated proteins and mRNAs of eIF4E3 and eIF4E4 in cells in exponential growth and in nutritional stress. Upon stress, eIF4G/eIF4A and PABP remain associated to the eIF4E, but the association with other 43S pre-initiation factors decreases, indicating that ribosome attachment is impaired. Most eIF4E3-associated mRNAs encode for metabolic proteins, while eIF4E4 associate to mRNAs encoding ribosomal proteins. Interestingly, for both eIF4E3/4, more mRNAs were associated in stressed cells than in non-stressed cells, even though stress causes ribosome disassembly. In summary, trypanosomes have two co-existing eIF4F complexes involved in translation of distinct mRNA cohorts important for growth. Under stress conditions, both complexes exit translation but remain bound to their mRNA targets.
 
Overall design We analysed RNAs associated to Trypanosoma cruzi eIF4E3 and eIF4E4 using RIP-seq in exponential growth and nutritional stress. We also analysed polyA total RNA and sequenced polysomal RNA (Ribo-seq). in these two conditions.
 
Contributor(s) Papini Gabiatti B, Ribeiro Freire E, Ferreira da Costa J, Galvão Ferrarini M, Reichert Assunção de Matos T, Preti H, Munhoz da Rocha I, Gomes Guimarães B, Kramer S, Ivo Tonin Zanchin N, Barbieri Holetz F
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Submission date Apr 11, 2024
Last update date Apr 14, 2024
Contact name Bernardo Papini Gabiatti
E-mail(s) bgabiatti@gmail.com
Organization name University of Würzburg
Street address Sanderring 2
City Würzburg
ZIP/Postal code 97070
Country Germany
 
Platforms (1)
GPL34379 Illumina HiSeq 2500 (Trypanosoma cruzi)
Samples (65)
GSM8198371 IgG (negative control) immunoprecipitation in exponential growth replicate 1
GSM8198372 IgG (negative control) immunoprecipitation in exponential growth replicate 2
GSM8198373 IgG (negative control) immunoprecipitation in exponential growth replicate 3
Relations
BioProject PRJNA1099224

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Supplementary file Size Download File type/resource
GSE263777_raw_counts.txt.gz 726.7 Kb (ftp)(http) TXT
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Raw data are available in SRA

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