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Series GSE263921 Query DataSets for GSE263921
Status Public on Apr 15, 2024
Title Profiling of exosomal microRNAs expression in culture medium of human esophageal cancer cell lines under hypoxic conditions
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The purpose of this analysis is to analyze the changes that occur in microRNAs contained in exosomes released from cancer cells in the tumor hypoxic environment of esophageal squamous cell carcinoma(ESCC).
We used microarrays to perform a comprehensive analysis of microRNAs in exosomes released from human ESCC cell lines under normoxic or hypoxic conditions.
 
Overall design Two human ESCC cell lines (KYSE-960, T.Tn) were cultured in 20% oxygen or 1% oxygen for 48 hours, and microRNA array analysis of RNA contained in exosomes extracted from the culture supernatant by ultracentrifugation was performed using the GeneChip miRNA 4.0 Array to identify common variable microRNA groups.
 
Contributor(s) Matsumoto Y, Abula M
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Submission date Apr 14, 2024
Last update date Apr 16, 2024
Contact name Yasunori Matsumoto
E-mail(s) ymatsumoto@chiba-u.jp
Organization name Chiba University
Department Frontier Surgery
Street address 1-8-1 Inohana, Chu-Ku
City Chiba-Shi
State/province Chiba
ZIP/Postal code 2608670
Country Japan
 
Platforms (1)
GPL21572 [miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version]
Samples (4)
GSM8207507 KYSE960 exosome normoxia
GSM8207508 KYSE960 exosome hypoxia
GSM8207509 T.Tn exosome normoxia
Relations
BioProject PRJNA1100195

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE263921_KYSE960_annotation.xlsx 6.6 Mb (ftp)(http) XLSX
GSE263921_RAW.tar 2.4 Mb (http)(custom) TAR (of CEL)
GSE263921_TTn_annotation.xlsx 6.7 Mb (ftp)(http) XLSX

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