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| Status |
Public on Jan 04, 2011 |
| Title |
Expression data of P4 stage hair follicle early bulge and non-bulge ORS cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Increasing evidence suggests that microRNAs may play important roles in regulating self-renewal and differentiation in mammalian stem cells (SCs). Here, we explore this issue in skin. We first characterize microRNA expression profiles of skin SCs versus their committed proliferative progenies and identify a microRNA subset associating with “stemness”. Of these, miR-125b is dramatically downregulated in early SC-progeny. We engineer an inducible mice system and show that when miR-125b is sustained in SC-progenies, tissue balance is reversibly skewed towards stemness at the expense of epidermal, oil-gland and HF differentiation. Using gain-and-loss of function in vitro, we further implicate miR-125b as a repressor of SC differentiation. In vivo, transcripts repressed upon miR-125b induction are enriched >700% for predicted miR-125b targets normally downregulated upon SC-lineage commitment. We verify some of these miR-125b targets, and show that Blimp1 and VDR in particular can account for many tissue imbalances we see when miR-125b is deregulated.
We used microarrays to compare the global gene expression profile of early bulge stem cells and non bulge ORS cells.
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| Overall design |
Hair follicle cells were isolated from P4 Backskin of K14-RFP/Sox9-EGFP double transgenic mice as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment. The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. Dead cells and large differentiated cells were excluded based on DAPI and side scattering. Early bulge cells were gated as GFPHi,RFPHi. Non-bulge ORS cells were gated as GFP-, RFPHi.
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| Contributor(s) |
Liang Z, Nicole S, Lisa P, Jason R, Jonathan N, Elaine F |
| Citation(s) |
21362569 |
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| Submission date |
Jan 03, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
liang zhang |
| E-mail(s) |
liangz@colorado.edu
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| Organization name |
university of colorado at boulder
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| Street address |
University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
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| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80309-0347 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE26396 |
Specific MicroRNAs Are Preferentially Expressed by Skin Stem Cells To Balance Self-Renewal and Early Lineage Commitment |
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| Relations |
| BioProject |
PRJNA142267 |
