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| Status |
Public on Apr 20, 2024 |
| Title |
mRNA profile of PANC-1 treated with CAF-derived Evs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Perineural invasion (PNI) is a unique biological feature of pancreatic cancer and is a key cause of pancreatic cancer metastasis, recurrence and poor postoperative survival, but its mechanism is largely unclarified. Clinical sample analysis and endoscopic ultrasonographic elasticity scoring indicated that cancer-associated fibroblasts (CAFs) are closely related to the occurrence of PNI. Furthermore, CAF-derived extracellular vesicles played an extremely important role in PNI in a dorsal root ganglion (DRG) coculture model and sciatic nerve model. Next, we demonstrated that CAFs promoted PNI via extracellular vesicle transmission of PNI-associated transcript (PIAT). To determine how CAF EVs exert its function, we performed mRNA sequencing on PANC-1 treated with CAFs-derived EVs
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| Overall design |
PANC-1 cells were pretreated with CAFs-derived Evs and collected for mRNA-seq.
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| Contributor(s) |
Zheng S, Hu C, Zhou Y, Chen R |
| Citation missing |
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| |
| Submission date |
Apr 15, 2024 |
| Last update date |
Apr 20, 2024 |
| Contact name |
Chonghui Hu |
| E-mail(s) |
huchonghui@gdph.org.cn
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| Organization name |
Guangdong Provincial People’s Hospital
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| Street address |
NA Zhongshan 2nd Road, Guangzhou
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| City |
Guangzhou |
| ZIP/Postal code |
5100080 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1100447 |
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