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| Status |
Public on Jun 30, 2024 |
| Title |
ChIP seq of CDK6-WT and CDK6-K43M in murine BCR-ABL(p185)+ pre/pro B-cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The aim of this experiment was to study the DNA interaction of a kinase-dead mutant of the cell cycle kinase CDK6 (CDK6-K43M) in a murine model of BCR-ABL driven leukemia.
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| Overall design |
ChIP seq was performed in mouse BCR-ABL(p185)+ Cdk6-/- pre/pro B cell lines transduced with either HA-tagged wildtype (CDK6-WT), or HA-tagged mutant (CDK6-K43M) CDK6. An antibody against HA was used to pull down the CDK6 variants and associated chromatin.Two replicates per genotype were analysed.
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| Contributor(s) |
Scheiblecker L, Klampfl T, Nebenführ S, Zojer M, Sexl V, Kollmann K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 17, 2024 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Thorsten Klampfl |
| E-mail(s) |
thorsten.klampfl@vetmeduni.ac.at
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| Organization name |
University of Veterinary Medicine Vienna
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| Street address |
Veterinaerplatz 1
|
| City |
Vienna |
| ZIP/Postal code |
1210 |
| Country |
Austria |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| GSM8214592 |
lymphoid cell line, HA-tagged CDK6-WT, replicate 1 |
| GSM8214593 |
lymphoid cell line, HA-tagged CDK6-WT, replicate 2 |
| GSM8214594 |
lymphoid cell line, HA-tagged CDK6-K43M, replicate 1 |
| GSM8214595 |
lymphoid cell line, HA-tagged CDK6-K43M, replicate 2 |
| GSM8214596 |
lymphoid cell line, Input, replicate 1 |
| GSM8214597 |
lymphoid cell line, Input, replicate 2 |
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| Relations |
| BioProject |
PRJNA1101496 |
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