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Series GSE264363 Query DataSets for GSE264363
Status Public on Apr 20, 2024
Title Gene expression profile at single cell level of hippocampal organoids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Hippocampal organoids are derived from human induced pluripotent stem cells under the guidance of multiple growth factors, such as proteins, chemical inhibitors, and activators. We used single-cell RNA sequencing (scRNA-seq) to analyze the transcriptomic signature of hippocampal organoids so that we could characterize cell identities in them. We prepared two single-cell samples from day-70 and day-81 hippocampal organoids.
 
Overall design We used 4 day-70 hippocampal organoids to prepare sample #1. Organoids were dissected into fragments and plated on flasks. We cultured them for 2-4 days, then isolated them with a Pierce Primary Neuron Isolation Kit with papain enzyme. We used 10 day-81 hippocampal organoids to prepare sample #2. Organoids were isolated directly with a Pierce Primary Neuron Isolation Kit with papain enzyme, broken up by pipetting up and down. The pre-sorting of live cells was completed using magnetic-activated cell sorting. The 3’ Gene expression Library was prepared with the quality-qualified cDNA based on Chromium Next GEM Single-cell 3’ GEM protocol. The quality of the library was assessed according to the standard protocol after fragmentation, adaptor ligation, and sample index PCR.
 
Contributor(s) Wu Y, Wen J
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Submission date Apr 18, 2024
Last update date Apr 20, 2024
Contact name Yan Wu
E-mail(s) 11955002@mail.sustech.edu.cn
Phone 18810461792
Organization name Southern University of Science and Technology
Department Department of biomedical engineering
Lab Xingyu Jiang
Street address Tao Yuan
City Shen Zhen
ZIP/Postal code 117549
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM8216858 hHO70
GSM8216859 hHO_81
Relations
BioProject PRJNA1102029

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Supplementary file Size Download File type/resource
GSE264363_RAW.tar 240.2 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA

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