|
| Status |
Public on Apr 20, 2024 |
| Title |
Gene expression profile at single cell level of hippocampal organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Hippocampal organoids are derived from human induced pluripotent stem cells under the guidance of multiple growth factors, such as proteins, chemical inhibitors, and activators. We used single-cell RNA sequencing (scRNA-seq) to analyze the transcriptomic signature of hippocampal organoids so that we could characterize cell identities in them. We prepared two single-cell samples from day-70 and day-81 hippocampal organoids.
|
| |
|
| Overall design |
We used 4 day-70 hippocampal organoids to prepare sample #1. Organoids were dissected into fragments and plated on flasks. We cultured them for 2-4 days, then isolated them with a Pierce Primary Neuron Isolation Kit with papain enzyme. We used 10 day-81 hippocampal organoids to prepare sample #2. Organoids were isolated directly with a Pierce Primary Neuron Isolation Kit with papain enzyme, broken up by pipetting up and down. The pre-sorting of live cells was completed using magnetic-activated cell sorting. The 3’ Gene expression Library was prepared with the quality-qualified cDNA based on Chromium Next GEM Single-cell 3’ GEM protocol. The quality of the library was assessed according to the standard protocol after fragmentation, adaptor ligation, and sample index PCR.
|
| |
|
| Contributor(s) |
Wu Y, Wen J |
| Citation(s) |
38744873 |
| |
| Submission date |
Apr 18, 2024 |
| Last update date |
May 30, 2024 |
| Contact name |
Yan Wu |
| E-mail(s) |
11955002@mail.sustech.edu.cn
|
| Phone |
18810461792
|
| Organization name |
Southern University of Science and Technology
|
| Department |
Department of biomedical engineering
|
| Lab |
Xingyu Jiang
|
| Street address |
Tao Yuan
|
| City |
Shen Zhen |
| ZIP/Postal code |
117549 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA1102029 |
