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| Status |
Public on Apr 26, 2024 |
| Title |
Understanding resistance to polymyxin and amikacin in Klebsiella pneumoniae |
| Organism |
Klebsiella pneumoniae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Klebsiella pneumoniae poses a significant global health threat primarily attributable to its pronounced resistance. Here, we report an in vitro acquired resistance analyses of K. pneumoniae to the combination of amikacin and polymyxin B. We found some differentially expressed genes associated with the resistome of K. pneumoniae. The main differences were found in the genes aphA, asmA, phoP, and in the arn operon. Once these genes are related to modification in lipopolysaccharides, aminoglycosides and in the membrane structure, the mechanisms associated with them can justify the resistance acquisition to amikacin and polymyxin b.
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| Overall design |
To investigate the resistance mechanism against amikacin and polymyxin B, we compare a resistant lineage (E6) and a control lineage (C1) after growing in subinhibitory concentrations of polymyxin B and amikacin for 1 hour. Subsequently, we conducted gene expression analysis utilizing RNA-seq data acquired from both lineages. Hence, a comparative gene expression profile of RNA-seq data for C1 and E6 lineages was obtained.
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| Contributor(s) |
de Souza C, Franco O |
| Citation missing |
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| BioProject |
PRJNA1100205 |
| |
| Submission date |
Apr 22, 2024 |
| Last update date |
Apr 26, 2024 |
| Contact name |
Camila Maurmann de Souza |
| Organization name |
Universidade Católica de Brasília
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| Department |
Biotechnology
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| Lab |
Centro de análises proteômicas e bioquímicas
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| Street address |
QS 07, Lote 01, Taguatinga Sul
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| City |
Brasília |
| State/province |
Distrito Federal |
| ZIP/Postal code |
71966-700 |
| Country |
Brazil |
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| Platforms (1) |
| GPL32198 |
NextSeq 550 (Klebsiella pneumoniae) |
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| Samples (6)
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