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Series GSE26499 Query DataSets for GSE26499
Status Public on Jan 07, 2012
Title Lineage-committed osteoclast precursors circulate in blood and settle down into bone
Organism Mus musculus
Experiment type Expression profiling by array
Summary Osteoclasts are derived from the monocyte/macrophage lineage, but little is known about osteoclast precursors in circulation. Bone marrow cells were subdivided into three populations; RANKhighFmslow, RANKhighFmshigh and RANKlowFmshigh. GeneChip analysis confirmed that the expression levels of monocyte-macrophage markers such as Emr1 (F4/80), Itgam (CD11b) and Csf1 (c-Fms) were lower in the RANKhighFmslow than RANKlowFmshigh population. In contrast, cells in the RANKhighFmslow population expressed higher levels of osteoclast markers such as Car ll (carbonic anhydrase ll), Mmp9 (matrix metalloproteinase 9), Acp5 (acid phosphatase 5) and Tfrc (transferrin receptor). These results suggest that RANKhighFmslow cells express few of the phenotypes of monocytes, and their differentiation into osteoclasts occurs at a slightly more advanced stage than that of the RANKlowFmshigh population.
 
Overall design RANKhighFmslow cells and RANKlowFmshigh cells were isolated from bone marrow in ddY mice by FACS (Fluorescent activated cell sorting). Differential expression levels of mRNA were determined by GeneChip analysis.
 
Contributor(s) Muto A, Mizoguchi T, Udagawa N, Ito S, Kawahara I, Abiko Y, Arai A, Harada S, Kobayashi Y, Nakamichi Y, Penninger JM, Noguchi T, Takahashi N
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Submission date Jan 07, 2011
Last update date Mar 04, 2019
Contact name Toshihide Mizoguchi
E-mail(s) toshim@po.mdi.ac.jp
Organization name Matsumoto Dental Univ.
Street address 1780 Gobara, Hiro-oka, Shiojiri
City Nagano
ZIP/Postal code 399-0781
Country Japan
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (2)
GSM651439 Bone marrow cells_RANKhighFmslow
GSM651440 Bone marrow cells_ RANKlowFmshigh
Relations
BioProject PRJNA136669

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26499_RAW.tar 8.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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