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| Status |
Public on Jun 15, 2005 |
| Title |
Intrinsic histone-DNA interactions and low nucleosome density are important for accessibility of promoters in yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by array
Genome binding/occupancy profiling by genome tiling array
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| Summary |
In yeast cells, preferential accessibility of the HIS3-PET56 promoter region is determined by a general property of the DNA sequence, not by defined sequence elements. In vivo, this region is largely devoid of nucleosomes, and accessibility is directly related to reduced histone density. The HIS3-PET56 and DED1 promoter regions associate poorly with histones in vitro, indicating that intrinsic nucleosome positioning and stability is a major determinant of preferential accessibility. Specific and genome-wide analyses indicate that low nucleosome density is a very common feature of yeast promoter regions that correlates poorly with transcriptional activation. Thus, the yeast genome is organized into structurally distinct promoter and non-promoter regions, whose DNA sequences inherently differ with respect to nucleosome formation. This organization ensures that transcription factors bind preferentially to appropriate sites in promoters, rather than to the excess of irrelevant sites in non-promoter regions.
Keywords: other
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| Contributor(s) |
Sekinger EA, Moqtaderi Z, Struhl K |
| Citation(s) |
15949447 |
| |
| Submission date |
May 13, 2005 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Kevin Struhl |
| E-mail(s) |
CBaisden@hms.harvard.edu
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| Phone |
617-432-2034
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| Fax |
617-432-2529
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| Organization name |
Harvard Medical School
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| Department |
Biological Chemistry and Molecular Pharmacology
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| Lab |
Struhl
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| Street address |
240 Longwood Ave.
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL2045 |
Saccharomyces cerevisiae intergenic regions |
| GPL2046 |
Saccharomyces cerevisiae ORFs |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA92129 |
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