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| Status |
Public on May 04, 2024 |
| Title |
Transcription factor OsWRKY11 induces rice heading at low concentrations but inhibits rice heading at high concentrations |
| Organism |
Oryza sativa |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To identify direct targets of OsWRKY11 in vivo, we performed chromatin immunoprecipitation sequencing (ChIP-seq).
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| Overall design |
The ChIP assays were conducted on shoots obtained from 3-week-old OsWRKY11-OE plants. A total of 2 g of seedling shoots from transgenic pUbi:OsWRKY11-Myc plants were harvested and fixed in a crosslinking solution containing 1% (w/v) formaldehyde under vacuum for 30 min on ice. Subsequently, crosslinking was terminated by treating with 0.125 mM glycine under vacuum for 5 min.
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| Contributor(s) |
Zhao L, Liu Y, Wang H, Yu D |
| Citation(s) |
38818952 |
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| Submission date |
Apr 29, 2024 |
| Last update date |
Aug 03, 2024 |
| Contact name |
Lirong Zhao |
| E-mail(s) |
lrzhao957@gmail.com
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| Organization name |
Chinese Academy of Sciences
|
| Department |
Xishuangbanna Tropical Botanical Garden
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| Street address |
No.88 Xuefu Road
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| City |
Kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650223 |
| Country |
China |
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| Platforms (1) |
| GPL23013 |
Illumina HiSeq 4000 (Oryza sativa) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1105855 |
