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| Status |
Public on May 05, 2024 |
| Title |
TEAD4 role in trophectoderm commitment and development is not conserved in non-rodent mammals |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The first lineage differentiation in mammals gives rise to the inner cell mass (ICM) and the trophectoderm (TE). In mice, TEAD4 is a master regulator of TE commitment, as it regulates the expression of other TE-specific genes and its ablation prevents blastocyst formation, but its role in other mammals remains unclear. TEAD4 ablation in bovine embryos did not impede TE differentiation or blastocyst formation, but a transcriptomic analysis was performed to see if there were any transcriptomic anomalies in knockout embryos.
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| Overall design |
RNAseq analysis was performed on five D8 in vitro produced wild type (WT) and four TEAD4 -/- (KO) bovine embryos generated by conventional CRISPR/Cas9 system.
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| Contributor(s) |
Pérez-Gómez A, González-Brusi L, Flores-Borobia I, Galiano-Cogolludo B, Lamas-Toranzo I, Hamze JG, Toledano-Díaz A, Santiago-Moreno J, Ramos-Ibeas P, Bermejo-Álvarez P |
| Citation missing |
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| |
| Submission date |
Apr 30, 2024 |
| Last update date |
May 05, 2024 |
| Contact name |
Leopoldo González-Brusi |
| Organization name |
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
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| Department |
Animal Reproduction
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| Street address |
Avda Puerta de Hierro, 12
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
| GPL23295 |
Illumina HiSeq 4000 (Bos taurus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1106536 |
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