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| Status |
Public on May 08, 2024 |
| Title |
Analysis of gene expression in SLMAP3 knockout immortalized E14.5 mouse embryonic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Due to the developmental abnormalities observed in SLMAP3 knockout mouse embryos, we aimed to investigated the impact of gene expression in E14.5 mouse embryonic fibroblasts (MEFs) isolated from these animals. The cells used for RNA-sequencing were previously immortalized with a transient expression of SV40 large T (SV40 1: pBSSVD2005 was a gift from David Ron - Addgene plasmid # 21826 ; http://n2t.net/addgene:21826 ; RRID:Addgene_21826). These immortalized cells express mostly the largest isoforms of SLMAP (~ 90kDa), which are the ones knocked out in the embryos, and therefore compensation because of other isoforms is negligible.
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| Overall design |
Immortalized mouse embryonic fibroblasts from 4 different wildtype and 4 different SLMAP3 knockout E14.5 embryos were used. RNA-sequencing was performed with each sample for the quantification of differential gene expression between the knockout and wild type cells.
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| Contributor(s) |
Tuana B, Dias AP, Rehmani T |
| Citation missing |
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| |
| Submission date |
May 05, 2024 |
| Last update date |
May 08, 2024 |
| Contact name |
Balwant Tuana |
| E-mail(s) |
btuana@uottawa.ca
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| Organization name |
University of Ottawa
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| Department |
Cellular and Molecular Medicine
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| Lab |
Dr. Balwant Tuana
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| Street address |
451 Smyth Road
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| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1H 8M5 |
| Country |
Canada |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1107953 |
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