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Series GSE2667 Query DataSets for GSE2667
Status Public on Nov 16, 2005
Title Genome-wide transcriptional analysis of the phosphate starvation stimulon of Bacillus subtilis
Organism Bacillus subtilis
Experiment type Expression profiling by array
Summary Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the acquisition of phosphate (Pi) from the environment and reducing the cellular requirement for this essential nutrient, the SigB regulon provide non-specific resistance to stress by protecting essential cellular components such as DNA and membranes. We have characterized the phosphate starvation stress response of B. subtilis at a genome-wide level using DNA macroarrays. A combination of outlier and cluster analyses identified putatively new members of the PhoP regulon, namely yfkN (2',3'-cyclic-nucleotide 2'-phosphodiesterase), yurI (ribonuclease), yjdB (unknown) and vpr (extracellular serine protease). YurI is thought to be responsible for the non-specific degradation of RNA, whilst the activity of YfkN on various nucleotide phosphates suggests that it could act on substrates liberated by YurI which produces 3` or 5` phosphoribonucleotides.
The putative new PhoP regulon members are either known or predicted to be secreted and are likely to be important for the recovery of inorganic phosphate from a variety of organic sources of phosphate in the environment.
Keywords: other
 
 
Contributor(s) Allenby NE, O’Connor N, Prágai Z, Ward AC, Wipat A, Harwood CR, Allenby NE
Citation(s) 16291680
Submission date May 16, 2005
Last update date Mar 16, 2012
Contact name Nicholas Allenby
E-mail(s) Colin.Harwood@ncl.ac.uk
Phone (+44) 191 2227708
Fax (+44) 191 2227736
URL http://www.ncl.ac.uk/biomed/staff/profile/colin.harwood
Organization name The University of Newcastle upon Tyne
Department Institute for Cell and Molecular Biosciences
Lab Prof. Colin Harwood
Street address Leech Building, Medical School
City Newcastle upon Tyne
State/province Tyne and Wear
ZIP/Postal code NE2 4HH
Country United Kingdom
 
Platforms (1)
GPL188 Sigma Genosys Panorama Bacillus subtilis Gene Array
Samples (39)
GSM49830 Wild-type Transition -3 hours
GSM49975 Wild-type Transition -0.5 hours
GSM49998 Wild-type Transition +2.5 hours
Relations
BioProject PRJNA92101

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