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| Status |
Public on May 07, 2024 |
| Title |
Echinatin alleviates inflammation and pyroptosis in hypoxic-ischemic brain damage by inhibiting TLR4/ NF-κB pathway |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hypoxic ischemic encephalopathy (HIE) is a primary cause of neonatal death and disabilities resulting from perinatal hypoxia. The progression of HI injury is closely associated with neuroinflammation. Therefore, suppressing inflammatory pathways is a promising therapeutic strategy for treating HIE. Echinatin (Ech) is a principal active component of glycyrrhiza, with anti-inflammatory and anti-oxidative effects, often combined with other herbs to exert effects of clearing heat and detoxifying. This study aimed to investigate the anti-inflammatory and neuroprotective effects of Ech on neonatal rats with hypoxic-ischemic brain damage and on PC12 cells induced by oxygen-glucose deprivation (OGD).
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| Overall design |
Seven-day-old SD rats were subjected to 2.5 hours of ischemia-hypoxia for transcriptome sequencing. The results showed that inflammatory genes were enriched in HI rats compared with normal rats, the results were verified by PCR and wb, and TLR4, MyD88 and NF-κB were identified as the key regulators.. In vitro, Ech reduced TLR4-related protein levels, inhibited nuclear translocation of NF-κB, reduced the expression of downstream inflammatory cytokines, and prevented pyroptosis and cell membrane destruction. These results suggest that Ech can reduce neuroinflammation and pyroptosis in HI injury by inhibiting the TLR4/NF-κB pathway, which is expected to be a potential candidate for the treatment of HIE.
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| Contributor(s) |
Tao X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 07, 2024 |
| Last update date |
May 07, 2024 |
| Contact name |
xiaoyue tao |
| E-mail(s) |
lzlprof2021@126.com
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| Phone |
15727819123
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| Organization name |
wenzhou medical university
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| Street address |
puxieshi road, Wenzhou, China
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| City |
wenzhou |
| State/province |
zhejiang |
| ZIP/Postal code |
325027 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1108675 |
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