|
| Status |
Public on Aug 10, 2024 |
| Title |
Spatial transcriptomics profile at single cell level of murine glioblastomas treated with and without anti-CSF-1R inhibitors. |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Anti-CSF-1R treatment results in rapid regression of murne proneural glioblastoma models. However, the microenvironment response to treatment results in fibrosis that can promote tumor recurrence. We investigated the single-cell transcriptional profile of murine glioblastomas before and after treatment with an anti-CSF-1R inhibitor to determine the cause of fibrotic treatment response.
|
| |
|
| Overall design |
Gliobastomas were induced in mice using the RCAS model system at 5-weeks of age. Mice were monitored by MRI over the next 6 weeks until tumors developed that were at least 30 mm3 in volume. 3 mice were sacraficed prior to the initiation of treatment. 9 mice were treated daily with 200 mg/kg BLZ945 (Novartis), 3 were sacraficed 7-days post treatment, 3 at 28-days, 3 after rebound. All brain tumor samples were harvested and immediately embedded for cryosectioning for In Situ spatial transcriptomics using the 10X Genomics Xenium platform.
|
| |
|
| Contributor(s) |
Watson S, Zomer A, Joyce J, Fournier N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 07, 2024 |
| Last update date |
Aug 12, 2024 |
| Contact name |
Nadine Fournier |
| E-mail(s) |
nadine.fournier@sib.swiss
|
| Organization name |
Agora Cancer Research Center & Swiss Institute of Bioinformatics
|
| Lab |
Translational Data Science Facility
|
| Street address |
Agora Cancer Research Center, Bugon 25A
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1000 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
|
| Samples (13)
|
|
| This SubSeries is part of SuperSeries: |
| GSE266971 |
Spatial and scRNAseq profiling of murine glioblastomas treated with and without anti-CSF-1R inhibitors |
|
| Relations |
| BioProject |
PRJNA1108863 |
Less...
More...