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| Status |
Public on May 20, 2024 |
| Title |
Cut & Tag sequencing exhibited the chromosome binding regions of transcription factor IRF5 within murine bone marrow derived macrophages |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
CUT&Tag technology represents a cutting-edge method for investigating protein-DNA interactions. Utilizing a hyperactive novel fusion enzyme (Hyperactive pG-Tn5 / pA-Tn5 Transposase), this technique, under antibody guidance, specifically targets and cleaves DNA sequences near target proteins. It boasts several advantages over traditional ChIP-Seq, including lower cell input requirements, enhanced signal-to-noise ratio, and improved repeatability. Employing this innovative approach, we examined the binding strength of interferon regulatory factor 5 in bone marrow-derived macrophages, and analyzed the biological behaviors of IRF5 following stimulation of these cells.
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| Overall design |
Cleavage Under Targets and Tagmentation (CUT&Tag) for the IRF5 binding regions (using anti-IRF5 antibody (abcam ab21689) in the murine bone marrow derived macrophages stimulated with CpG or control
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 14, 2024 |
| Last update date |
May 20, 2024 |
| Contact name |
Zhejun Cai |
| E-mail(s) |
caizhejun@zju.edu.cn
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| Organization name |
Zhejiang University School of Medicine
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| Department |
Department of Cardiology, The Second Affiliated Hospital
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| Street address |
88 Jiefang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310009 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1111412 |
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