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Series GSE267400 Query DataSets for GSE267400
Status Public on May 20, 2024
Title Cut & Tag sequencing exhibited the chromosome binding regions of transcription factor IRF5 within murine bone marrow derived macrophages
Organism Mus musculus
Experiment type Other
Summary CUT&Tag technology represents a cutting-edge method for investigating protein-DNA interactions. Utilizing a hyperactive novel fusion enzyme (Hyperactive pG-Tn5 / pA-Tn5 Transposase), this technique, under antibody guidance, specifically targets and cleaves DNA sequences near target proteins. It boasts several advantages over traditional ChIP-Seq, including lower cell input requirements, enhanced signal-to-noise ratio, and improved repeatability. Employing this innovative approach, we examined the binding strength of interferon regulatory factor 5 in bone marrow-derived macrophages, and analyzed the biological behaviors of IRF5 following stimulation of these cells.
Overall design Cleavage Under Targets and Tagmentation (CUT&Tag) for the IRF5 binding regions (using anti-IRF5 antibody (abcam ab21689) in the murine bone marrow derived macrophages stimulated with CpG or control
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Submission date May 14, 2024
Last update date May 20, 2024
Contact name Zhejun Cai
Organization name Zhejiang University School of Medicine
Department Department of Cardiology, The Second Affiliated Hospital
Street address 88 Jiefang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310009
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM8265203 Control_1
GSM8265204 Control_1_IgG
GSM8265205 Control_2
BioProject PRJNA1111412

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE267400_CpG.peak_annotation.txt.gz 8.6 Mb (ftp)(http) TXT
GSE267400_CpG_IgG_1.tdf 220.0 Mb (ftp)(http) TDF
GSE267400_Ctrl.peak_annotation.txt.gz 14.9 Mb (ftp)(http) TXT
GSE267400_Ctrl_IgG_2.tdf 200.7 Mb (ftp)(http) TDF
GSE267400_RAW.tar 1019.1 Mb (http)(custom) TAR (of TDF, TXT)
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Raw data are available in SRA

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