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| Status |
Public on Jun 14, 2024 |
| Title |
A system for functional studies of the major virulence factor of malaria parasites |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The major virulence factor of Plasmodium falciparum parasites, PfEMP1 is expressed by a multigene family, termed var genes. Here selection linked integration (SLI) was utilized to modify var genes in P. falciparum parasites to select for parasite populations expressing a single var gene. Bulk RNA was isolated from ring stage parasites of these SLI parasite populations and analyzed with next generation sequencing. The proportion of exon 2 transcripts of var genes normalized to transcripts per million was determined per cell line to confirm the predominant expression of the desired var gene.
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| Overall design |
RNA-seq samples of Plasmodium falciparum infected human erythorcyes
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| Contributor(s) |
Cronshagen J, Allweier J, Mesén-Ramírez P, Stäcker J, Viktoria Vaaben A, Ramón-Zamorano G, Naranjo I, Ofori S, Jansen PW, Hornebeck J, Kieferle F, Martin E, Murk A, Castro-Peña C, Bártfai R, Lavstsen T, Bruchhaus I, Spielmann T |
| Citation missing |
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| |
| Submission date |
May 14, 2024 |
| Last update date |
Jun 14, 2024 |
| Contact name |
Johannes Allweier |
| E-mail(s) |
johannes.allweier@bnitm.de
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| Organization name |
Bernhard Nocht Institute for Tropical Medicine
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| Street address |
Bernhard-Nocht-Straße 74
|
| City |
Hamburg |
| ZIP/Postal code |
20359 |
| Country |
Germany |
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| Platforms (1) |
| GPL26920 |
NextSeq 550 (Plasmodium falciparum) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA1111452 |
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