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| Status |
Public on May 22, 2024 |
| Title |
Microglia are not necessary for maintenance of blood-brain barrier properties in health, but PLX5622 alters brain endothelial cholesterol metabolism (Liver Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Microglia are resident immune cells of the central nervous system, yet their functions far exceed those related to immunology. From pruning neural synapses during development to preventing excessive neural activity throughout life, microglia are intimately involved in the brain’s most basic processes. Studies have reported a close interaction between microglia and endothelial cells, as well as both helpful and harmful roles for microglia at the blood-brain barrier (BBB) in the context of disease. However, much less work has been done to understand microglia-endothelial cell interactions in the healthy brain. Here, we aim to determine the role of microglia at the healthy BBB. We used the colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622 to deplete microglia and analyzed BBB ultrastructure, permeability, and transcriptome. Interestingly, we found that, despite their direct contact with endothelial cells, microglia are not necessary for maintenance of BBB structure, function, or gene expression in the healthy brain. However, we found that PLX5622 treatment alters brain endothelial cholesterol metabolism, and this effect was independent from microglial depletion, suggesting PLX5622 has off-target effects on brain vasculature.
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| Overall design |
We aimed to determine whether PLX5622 affects cholesterol metabolism in peripheral endothelial cells (in addition to central nervous system endothelial cells). We put mice on control or PLX5622 diet for one month and then acutely isolated liver endothelial cells for RNA sequencing.
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| Contributor(s) |
Profaci CP, Harvey SS, Daneman R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 15, 2024 |
| Last update date |
May 22, 2024 |
| Contact name |
Caterina Profaci |
| Organization name |
University of California, San Diego
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| GSM8269479 |
control diet, liver endothelial cells, biol rep 1 |
| GSM8269480 |
control diet, liver endothelial cells, biol rep 2 |
| GSM8269481 |
control diet, liver endothelial cells, biol rep 3 |
| GSM8269482 |
PLX5622 diet, liver endothelial cells, biol rep 1 |
| GSM8269483 |
PLX5622 diet, liver endothelial cells, biol rep 2 |
| GSM8269484 |
PLX5622 diet, liver endothelial cells, biol rep 3 |
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| Relations |
| BioProject |
PRJNA1111909 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267594_PLX_Liver_Seq.xlsx |
3.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
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