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| Status |
Public on May 18, 2024 |
| Title |
Transcriptomics profile of adipose and adipocyte with Gls perturbation |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We generated single nucleus transcriptomic atlas for white and brown adipose tissue from adipocyte-specific Gls knockout mouse, and also generated bulk RNA-seq from in vitro differentiated human white adipocytes with/without GLS knock down.
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| Overall design |
For snRNA-seq, brown and white adipose tissues were isolated from adipocyte-specific Gls knockout mice and Cre- neagtive Littermates and flash frozen in liquid nitrogen. The nuclei was extracted and performed snRNA-seq. For bulk RNA-seq, in vitro differentiated white adipocytes where transfected eight days post beginning of differentiation, with scrumble or GLS targeting siRNA, cells were harvested and total RNA extracted at D13 of differentiation.
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| Contributor(s) |
Zhong J, Jalkanen J, Massier L, Renzi G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 17, 2024 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Jiawei Zhong |
| Organization name |
Karolinska Institutet
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| Department |
Department of Medicine, Huddinge
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| Lab |
Mikael Rydén & Niklas Mejhert lab
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| Street address |
Blickagången 16, Flemingsberg
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| City |
Stockholm |
| ZIP/Postal code |
14182 |
| Country |
Sweden |
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| Platforms (2) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1112901 |
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