|
| Status |
Public on Jan 25, 2011 |
| Title |
Mast cell microRNAs regulated by KIT signaling |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Objective is to identify microRNAs regulated by KIT signaling in mast cells. miRNA profiles of murine bone marrow derived mast cells with and without SCF treatment were compared. In addition, miRNA profiles of the P815 mutant KIT cell line with and without the KIT inhibitor, imatinib, were compared. We identified miRNAs that were differentially regulated by KIT signals.
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| Overall design |
Two systems were used to identify microRNAs regulated by KIT. RNA was collected from primary murine bone marrow derived mast cells (BMMC) treated and not treated with KIT ligand (SCF). In addition, RNA was harvested from the P815 (activating KIT mutation positive) cell line treated and not treated with the KIT inhibitor, imatinib. Samples were harvested after 24 hours of treatment
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| |
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| Citation(s) |
21273305 |
| |
| Submission date |
Jan 21, 2011 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Cliff Takemoto |
| E-mail(s) |
ctakemot@jhmi.edu
|
| Phone |
410 955-6132
|
| Fax |
410 955-8208
|
| Organization name |
The Johns Hopkins University
|
| Department |
Pediatric Hematology
|
| Street address |
720 Rutland Ave/Ross 1125
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8573 |
Custom microRNA microarray Design 4343 |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA136253 |
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