 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 21, 2024 |
| Title |
Mapping spatially resolved transcriptomes in human and mouse pulmonary fibrosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis and limited treatment options. Efforts to identify effective treatments are thwarted by limited understanding of IPF pathogenesis and poor translatability of available preclinical models. To address these limitations, we generated spatially resolved transcriptome maps of human IPF and bleomycin-induced mouse lung fibrosis.
|
| |
|
| Overall design |
Female C57BL/6NCrl mice were challenged with 30 μl saline or bleomycin (Apollo Scientific, BI3543, Chemtronica Sweden; 40 µg/mouse) dissolved in saline via oropharyngeal route administration. Lungs were collected at day 7 (n=6 BLM, n=3 vehicle) or day 21 (n=6 BLM, n=3 vehicle) following administration. The mice were purchased from Charles River (Germany), and were 8 weeks + 5 days old at the time of study initiation. Animal handling conformed to standards established by the Council of Europe ETS123 AppA, the Helsinki Convention for the Use and Care of Animals, Swedish legislation, and AstraZeneca global internal standards. All mouse experiments were approved by the Gothenburg Ethics Committee for Experimental Animals in Sweden and conformed to Directive 2010/63/EU. The present study was approved by the local Ethical committee in Gothenburg (EA000680-2017) and the approved site number is 31-5373/11.
|
| |
|
| Contributor(s) |
Franzén L, Olsson Lindvall M, Hühn M, Ptasinski V, Setyo L, Keith BP, Collin A, Oag S, Volckaert T, Borde A, Lundeberg J, Lindgren J, Belfield G, Jackson S, Ollerstam A, Stamou M, Ståhl PL, Hornberg JJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 20, 2024 |
| Last update date |
Jun 03, 2024 |
| Contact name |
Benjamin Keith |
| E-mail(s) |
ben.keith@astrazeneca.com
|
| Organization name |
AstraZeneca
|
| Street address |
Pepparedsleden 1
|
| City |
Mölndal |
| ZIP/Postal code |
43183 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (24)
|
| GSM8281387 |
d7, bleo, 1 |
| GSM8281388 |
d7, bleo, 2 |
| GSM8281389 |
d7, bleo, 3 |
| GSM8281390 |
d7, bleo, 4a |
| GSM8281391 |
d7, bleo, 5a |
| GSM8281392 |
d7, bleo, 6a |
| GSM8281393 |
d7, bleo, 4b |
| GSM8281394 |
d7, bleo, 5b |
| GSM8281395 |
d7, bleo, 6b |
| GSM8281396 |
d21, ctrl, 1 |
| GSM8281397 |
d21, ctrl, 2 |
| GSM8281398 |
d21, ctrl, 3 |
| GSM8281399 |
d21, bleo, 1 |
| GSM8281400 |
d21, bleo, 2 |
| GSM8281401 |
d21, bleo, 3 |
| GSM8281402 |
d21, bleo, 4a |
| GSM8281403 |
d21, bleo, 5a |
| GSM8281404 |
d21, bleo, 6a |
| GSM8281405 |
d21, bleo, 4b |
| GSM8281406 |
d21, bleo, 5b |
| GSM8281407 |
d21, bleo, 6b |
|
| Relations |
| BioProject |
PRJNA1113613 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267904_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of CSV, JPG, JSON, MTX, PNG, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...