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Status |
Public on Jan 26, 2011 |
Title |
Mating-type specific gene expression in the filamentous ascomycete Neurospora tetrasperma |
Platform organism |
Neurospora crassa |
Sample organisms |
Neurospora crassa; Neurospora tetrasperma |
Experiment type |
Expression profiling by array
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Summary |
Neurospora tetrasperma is a pseudohomothallic filamentous ascomycete with a large (~ 7 Mbp) region of suppressed recombination surrounding its mating-type (mat) locus. The suppressed recombination has lead to sequence divergence between the two mating-type chromosomes of wild-type heterokaryotic strains, while the remaining genome is largely homoallelic. In this study, we use microarray technology to manifest expression divergence linked to mating type in N. tetrasperma. N. tetrasperma and N. crassa, were grown on agar regimes inducing sexual growth (Synthetic Crossing medium) and vegetative growth (Vogel's Medium), respectively.
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Overall design |
[SC]: Neurospora tetrasperma mat-A FGSC#1270; mat-a FGSC#1271; Mat-A FGSC#9033; mat-a FGSC#9034; N. crassa mat-A FGSC#2489 and mat-a FGSC 4200: Synthetic Crossing medium was used as a nutrient regime before sampling and processing
[Veg]: Neurospora tetrasperma mat-A FGSC#1270; mat-a FGSC#1271; Mat-A FGSC#9033; mat-a FGSC#9034; N. crassa mat-A FGSC#2489 and mat-a FGSC 4200: Vogel's Medium (Vegetative Medium) was used as a nutrient regime before sampling and processing
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Contributor(s) |
Samils N, Karlsson M, Wang Z, Kasuga T, Bastiaans E, Townsend JP, Johannesson H |
Citation missing |
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Submission date |
Jan 21, 2011 |
Last update date |
Oct 21, 2013 |
Contact name |
Nicklas Samils |
E-mail(s) |
nicklas.samils@ebc.uu.se
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Organization name |
Uppsala University
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Department |
Evolutionary Biology
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Street address |
NorbyƤgen 18D
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City |
Uppsala |
ZIP/Postal code |
75236 Uppsala |
Country |
Sweden |
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Platforms (1) |
GPL10640 |
Yale University Neurospora crassa 70-mer oligo whole genome transcription array |
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Samples (36)
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Relations |
BioProject |
PRJNA136275 |