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| Status |
Public on May 29, 2024 |
| Title |
Human airway epithelial cells co-treated with IL-4 and IL-13 for seven days. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Nasal basal epithelial cells from 6 healthy adult donors were grown in air-liquid interface (ALI) cultures for fourteen days. Cells were then treated with 10 ng/mL of IL-4 and 10 ng/mL of IL-13 for seven additional days. Next, cell lysates were used for low-input RNA-seq library preparation (Smart-seq2) and sequencing.
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| Overall design |
To investigate the effect of cytokine stimulation on epithelial cells we generated gene expression profiles using RNA-seq from 5 healthy donors under in vitro co-stimulation with IL-4 and IL-13 or under resting condition.
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| Contributor(s) |
Barrett N, Gutierrez-Arcelus M, Hallen N, Huang G, Djeddi S, Fernandez-Salinas D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 21, 2024 |
| Last update date |
Jun 17, 2024 |
| Contact name |
Nora Barrett |
| Organization name |
Brigham and Women’s Hospital
|
| Department |
Allergy and Clinical Immunology
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| Lab |
Barrett Lab
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| Street address |
60 Fenwood Rd
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| City |
Boston |
| State/province |
Massachussetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| GSM8285471 |
AECs co-stimulated in vitro with IL-4 and IL-13, Subject 1 |
| GSM8285472 |
AECs co-stimulated in vitro with IL-4 and IL-13, Subject 2 |
| GSM8285473 |
AECs co-stimulated in vitro with IL-4 and IL-13, Subject 3 |
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| Relations |
| BioProject |
PRJNA1114125 |
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