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| Status |
Public on May 26, 2024 |
| Title |
Transcriptome Analysis Reveals Potential Protective Effects and Biomarkers of Pien Tze Huang on CCl4-induced Liver Cancer (miRNA) |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Pien Tze Huang (PZH) is a traditional Chinese medicinal formula that has been extensively used in Asia for the treatment of liver diseases. We created two groups of liver cancer mouse models induced by carbon tetrachloride (CCl4): one group received treatment with PZH and the other did not receive any treatment. In the non-PZH treated group, we observed a significant increase in liver function indices including albumin (ALB), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Histological examination using hematoxylin and eosin (HE) staining revealed inflammation around the hepatic vein in the non-PZH treated group. Furthermore, Masson's trichrome staining demonstrated the presence of blue-stained collagen fibers around the hepatic vein in the non-PZH treated group. These findings suggest that PZH may have therapeutic effects on liver inflammation and cancer.
To further explore the molecular mechanisms of PZH's action, we conducted mRNA, long non-coding RNA (lncRNA), circular RNA (circRNA), and microRNA (miRNA) sequencing to identify potential drug targets at the transcriptome level. We identified 478 differentially expressed protein-coding transcripts and 68 differentially expressed lncRNA transcripts. Our study provides insights into potential transcriptional therapeutic targets for the clinical use of PZH in treating liver diseases.
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| Overall design |
We construct two groups liver cancer mice model using CCl4, one group using PZH treatment, and the other did not receive any treatment. We also did a blank experiment with normal mice using water.In the 22nd week, we selected six mice from each group for miRNA sequencing.
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| Contributor(s) |
Wang Y, Wu H, Qin S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 24, 2024 |
| Last update date |
May 27, 2024 |
| Contact name |
Yongzhi Wang |
| Organization name |
Shanghai Jiao Tong University
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| Street address |
Huashan Road 1954
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE268338 |
Transcriptome Analysis Reveals Potential Protective Effects and Biomarkers of Pien Tze Huang on CCl4-induced Liver Cancer |
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| Relations |
| BioProject |
PRJNA1116233 |
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