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| Status |
Public on Sep 01, 2024 |
| Title |
Bulk RNAseq of FNE1 cells with ectopic expression of YAP1 and HPV E6/E7 oncogenes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
High grade serous ovarian carcinoma (HGSOC) is the most common and aggressive ovarian malignancy. Accumulating evidence indicates that HGSOC may originate from human fallopian tube epithelial cells (FTECs), although the exact pathogen(s) and/or molecular mechanism underlying the malignant transformation of FTECs is unclear. Here we show that human papillomavirus (HPV), which could reach FTECs via retrograde menstruation or sperm-carrying, interacts with the yes-associated protein 1 (YAP1) to drive the initiation and progression of high grade serous cancer (HGSC). HPV prevents FTECs from natural replicative and YAP1-induced senescence, thereby promoting YAP1-induced malignant Transformation of FTECs. HPV also stimulates proliferation and drives metastasis of YAP1-transformed FTECs. YAP1, in turn, stimulates the expression of the putative HPV receptors and suppresses the innate immune system to facilitate HPV acquisition. These findings provide critical clues for developing new strategies to prevent and treat HGSOC.
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| Overall design |
FNE1 cells were cultured to 40% confluent and then transfected with retrovirus-based empty control vector (MXIV), or vectors expressing wild type of YAP1 (YAP), or constitutively active YAP1 (YAPS127A, a replacement of Serine at residue 127 with Alanine resulting in the constitutive activation of YAP1 protein [66]). All transfected cells were selected with G418 (200-400μg/ml). YAP1 expression in these cells was confirmed by RT-PCR and Western blot.
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| Contributor(s) |
Liu J, He C, Lv X, Wang C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 31, 2024 |
| Last update date |
Sep 01, 2024 |
| Contact name |
Jiyuan Liu |
| E-mail(s) |
JLIU78@MGH.HARVARD.EDU
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| Organization name |
Mass Gen Hospital
|
| Street address |
55 fruit st.
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| City |
boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| GSM8300990 |
FNE1 cells transfected with MXIV backbones_rep1 |
| GSM8300991 |
FNE1 cells transfected with MXIV backbones_rep2 |
| GSM8300992 |
FNE1 cells transfected with MXIV backbones_rep3 |
| GSM8300993 |
FNE1 cells overexpressed with YapS127A_rep1 |
| GSM8300994 |
FNE1 cells overexpressed with YapS127A_rep2 |
| GSM8300995 |
FNE1 cells overexpressed with YapS127A_rep3 |
| GSM8300996 |
FNE1 cells overexpressed with E6E7_rep1 |
| GSM8300997 |
FNE1 cells overexpressed with E6E7_rep2 |
| GSM8300998 |
FNE1 cells overexpressed with E6E7_rep3 |
| GSM8300999 |
FNE1 cells overexpressed with E6E7 and YapS127A_rep1 |
| GSM8301000 |
FNE1 cells overexpressed with E6E7 and YapS127A_rep2 |
| GSM8301001 |
FNE1 cells overexpressed with E6E7 and YapS127A_rep3 |
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| Relations |
| BioProject |
PRJNA1118818 |
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