 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 08, 2024 |
| Title |
scRNA-Seq reveals anti-fibrotic mechanisms of TRPC6 inhibition [Spatial Transcriptomics] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Inhibition of transient receptor potential canonical 6 channels (TRPC6) alleviates tubular injury and renal fibrosis induced by unilateral ureteral obstruction (UUO).However, the exact renoprotective mechanisms are unknown. In this study, we utilized single-cell RNA sequencing (scRNA-Seq) to define the cellular and transcriptional landscape associated with renoprotection through in vivo TRPC6 inhibition, specifically using SH045 (larixyl N-methylcarbamate), in the UUO model.
|
| |
|
| Overall design |
Six C57BL/6 mice underwent unilateral ureteral obstruction (UUO) surgery. Seven days post-surgery, the kidneys from Vehicle-treated (n=3) and SH045-treated (n=3) mice were harvested. The contralateral kidneys from Vehicle-treated mice (n=3) served as the control group (Ctrl group). A total of nine kidney samples were used for single-cell RNA sequencing (scRNA-seq). Additionally, two of these samples (one from the SH045 group and one from the Vehicle group) were simultaneously analyzed using spatial transcriptomics.
|
| |
|
| Contributor(s) |
Xu Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 04, 2024 |
| Last update date |
Jun 08, 2024 |
| Contact name |
Yao Xu |
| E-mail(s) |
yao.xu@med.uni-greifswald.de
|
| Organization name |
University Medicine Greifswald
|
| Street address |
Felix-Hausdorff-Straße 3
|
| City |
Greifswald |
| State/province |
State |
| ZIP/Postal code |
17487 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA1120043 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269063_RAW.tar |
33.1 Mb |
(http)(custom) |
TAR (of CSV, JPG, JSON, MTX, PNG, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
