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Status |
Public on Jul 31, 2024 |
Title |
Gene expression profile of single endocardial cells in 48 and 72 hpf zebrafish embryos/larvae. In the study "The heart is a niche for hematopoietic stem and progenitor cells in zebrafish." |
Organism |
Danio rerio |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We used single-cell RNA expression to uncover the cellular and molecular heterogeneity of isolated endocardial cells in the heart. From this study, we uncovered the presence of an endocardial-hematopoietic cluster, along with other significant clusters including valve endocardial and interstitial cells, and non-valve cells.
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Overall design |
Endocardial cells were isolated using FACS from physically extracted Tg(kdrl:nls-mCherry) and Tg(cd41:GFP) hearts according to presence of mCherry-positive cells, and further analyzed using scRNAseq.
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Contributor(s) |
Bornhorst D, Hejjaji AV, Steuter L, Woodhead N, Maier P, Gentile A, Alhajkadour A, Larrain OS, Weber M, Kikhi K, Huisken J, Tamplin OJ, Stainier DY, Günther S, Gunawan F |
Citation(s) |
39217144 |
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Submission date |
Jun 07, 2024 |
Last update date |
Oct 01, 2024 |
Contact name |
Stefan Günther |
E-mail(s) |
stefan.guenther@mpi-bn.mpg.de
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Organization name |
MPI for heart and lung research
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Street address |
ludwigtr. 43
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City |
bad nauheim |
ZIP/Postal code |
61231 |
Country |
Germany |
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Platforms (1) |
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Samples (2) |
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Relations |
BioProject |
PRJNA1121299 |
Supplementary file |
Size |
Download |
File type/resource |
GSE269378_RAW.tar |
46.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
Raw data are available in SRA |
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