|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Jun 13, 2024 |
Title |
8-Oxoguanine DNA glycosylase 1 hijacked by nucleoprotein conceals guanine oxidation in respiratory syncytial virus RNA |
Organism |
Homo sapiens |
Experiment type |
Other
|
Summary |
Respiratory Syncytial Virus (RSV), alongside other prominent respiratory RNA viruses such as influenza and SARS-CoV-2, significantly contributes to the global incidence of respiratory tract infections. These pathogens prompt the production of reactive oxygen species (ROS), which play a crucial role in the onset and progression of respiratory diseases. However, the strategies by which viral RNA manages ROS-induced base oxidation are not well understood. Here we uncover that 8-oxo-7,8-dihydroguanine (8-oxoGua) is not merely an incidental byproduct of ROS activity but serves as a strategic adaptation of RSV RNA during replication within host cells. Through RNA immunoprecipitation and next-generation sequencing, we discovered that 8-oxoguanine DNA glycosylase 1 (OGG1) binding sites are predominantly found in the RSV antigenome, especially within guanine-rich areas. Further investigation revealed that viral ribonucleoprotein complexes specifically hijack OGG1. Crucially, our findings show that inhibiting OGG1's ability to recognize 8-oxoGua leads to a substantial reduction in RSV progeny production. Our results underscore the viral replication machinery's adaptation to oxidative challenges, pointing to the inhibition of OGG1's reading function as a potential novel strategy for antiviral intervention.
|
|
|
Overall design |
RSV nucleocapsid immunoprecipitation RNA-sequencing (RNA-seq) for OGG1 in Human Small Airway Epithelial Cells (hSAECs).
|
|
|
Contributor(s) |
Pan L, Boldogh I |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Jun 13, 2024 |
Last update date |
Jun 14, 2024 |
Contact name |
Haiping Hao |
E-mail(s) |
hahao@UTMB.EDU
|
Phone |
4097726349
|
Organization name |
University of Texas Medical Branch
|
Department |
Biochemistry and Molecular Biology
|
Street address |
1005 strand st
|
City |
Galveston |
State/province |
Texas |
ZIP/Postal code |
77555 |
Country |
USA |
|
|
Platforms (1) |
GPL22790 |
Illumina MiniSeq (Homo sapiens) |
|
Samples (4)
|
|
Relations |
BioProject |
PRJNA1123660 |
Supplementary file |
Size |
Download |
File type/resource |
GSE269769_FLAG-Ab-vs-Input-cell-hg38-enriched.bedgraph.gz |
4.7 Mb |
(ftp)(http) |
BEDGRAPH |
GSE269769_OGG1-Ab-vs-Input-cell-hg38-enriched.bedgraph.gz |
4.2 Mb |
(ftp)(http) |
BEDGRAPH |
GSE269769_RAW.tar |
570.0 Kb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
Raw data are available in SRA |
|
|
|
|
|