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| Status |
Public on Jun 13, 2024 |
| Title |
8-Oxoguanine DNA glycosylase 1 hijacked by nucleoprotein conceals guanine oxidation in respiratory syncytial virus RNA |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Respiratory Syncytial Virus (RSV), alongside other prominent respiratory RNA viruses such as influenza and SARS-CoV-2, significantly contributes to the global incidence of respiratory tract infections. These pathogens prompt the production of reactive oxygen species (ROS), which play a crucial role in the onset and progression of respiratory diseases. However, the strategies by which viral RNA manages ROS-induced base oxidation are not well understood. Here we uncover that 8-oxo-7,8-dihydroguanine (8-oxoGua) is not merely an incidental byproduct of ROS activity but serves as a strategic adaptation of RSV RNA during replication within host cells. Through RNA immunoprecipitation and next-generation sequencing, we discovered that 8-oxoguanine DNA glycosylase 1 (OGG1) binding sites are predominantly found in the RSV antigenome, especially within guanine-rich areas. Further investigation revealed that viral ribonucleoprotein complexes specifically hijack OGG1. Crucially, our findings show that inhibiting OGG1's ability to recognize 8-oxoGua leads to a substantial reduction in RSV progeny production. Our results underscore the viral replication machinery's adaptation to oxidative challenges, pointing to the inhibition of OGG1's reading function as a potential novel strategy for antiviral intervention.
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| Overall design |
RSV nucleocapsid immunoprecipitation RNA-sequencing (RNA-seq) for OGG1 in Human Small Airway Epithelial Cells (hSAECs).
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| Contributor(s) |
Pan L, Boldogh I |
| Citation missing |
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| Submission date |
Jun 13, 2024 |
| Last update date |
Jun 14, 2024 |
| Contact name |
Haiping Hao |
| E-mail(s) |
hahao@UTMB.EDU
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| Phone |
4097726349
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| Organization name |
University of Texas Medical Branch
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| Department |
Biochemistry and Molecular Biology
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| Street address |
1005 strand st
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| City |
Galveston |
| State/province |
Texas |
| ZIP/Postal code |
77555 |
| Country |
USA |
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| Platforms (1) |
| GPL22790 |
Illumina MiniSeq (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1123660 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269769_FLAG-Ab-vs-Input-cell-hg38-enriched.bedgraph.gz |
4.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE269769_OGG1-Ab-vs-Input-cell-hg38-enriched.bedgraph.gz |
4.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE269769_RAW.tar |
570.0 Kb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
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