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| Status |
Public on Jun 17, 2024 |
| Title |
Expression Patterns of Mal genes and Association with Differential Maltose and Maltotriose Transport rate of Two Saccharomyces pastorianus Yeasts |
| Organism |
Saccharomyces pastorianus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Beer brewing is a well-known process that still faces great challenges, such as the total consumption of sugars present in the fermentation media. Lager-style beer, major worldwide beer type, is elaborated by Saccharomyces pastorianus (Sp) yeast which must ferment high maltotriose content worts, but its consumption represents a notable problem, especially among Sp strains belonging to group I. Factors like fermentation conditions, presence of maltotriose transporters, transporter copy number variation, and genetic regulation variations contribute to this issue. We assess the factors affecting fermentation in two Sp yeast strains: SpIB1, with limited maltotriose uptake, and SpIB2, known for efficient maltotriose transport. Here, SpIB2 transported significantly more maltose (28%) and maltotriose (32%) compared to SpIB1. Furthermore, SpIB2 expressed all MAL transporters (ScMALx1, SeMALx1, ScAGT1, SeAGT1, MTT1, and MPHx) on the first day of fermentation, while SpIB1 only exhibited ScMalx1, ScAGT1, and MPH2/3 genes. Some SpIB2 transporters had polymorphic transmembrane domains (TMD) resembling MTT1, accompanied by higher expression of these transporters and its positive regulator genes like MAL63. These findings suggest that, in addition of the factors mentioned above, positive regulators of Mal transporters contribute significatively to phenotypic diversity in maltose and maltotriose consumption among the studied lager yeast strains.
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| Overall design |
The goal is to assess the underling genes involved in the phenotypic diversity of maltose and maltotriose consumption of two lager yeast strains (SpIB1 and SpIB2). Both yeasts were subjected to lager fermentation in a standard wort for 5 days and samples were collected during day 2 and 5.
We then performed a gene expression analysis using the RNA-seq data from both yeasts on days 2 and 5 of fermentation.
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| Web link |
https://journals.asm.org/doi/10.1128/aem.00397-24
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| Contributor(s) |
Pereyra Alférez B, García JH, Hernández Vásquez CI, del Rocio Pérez Ortega E, Damas Buenrostro LC, Martzinez Segundo AG |
| Citation(s) |
38975758 |
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| Submission date |
Jun 14, 2024 |
| Last update date |
Nov 06, 2024 |
| Contact name |
Benito Pereyra Alférez |
| E-mail(s) |
benito.pereyraal@uanl.edu.mx, cesar.hernandezvsqz@uanl.edu.mx
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| Organization name |
Universidad Autónoma de Nuevo León
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| Department |
Instituto de Biotecnología
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| Lab |
Laboratorio 4
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| Street address |
Av. Pedro de Alba S/N
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| City |
San Nicolás de los Garza |
| State/province |
Nuevo León |
| ZIP/Postal code |
66455 |
| Country |
Mexico |
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| Platforms (1) |
| GPL34596 |
Illumina HiSeq 2500 (Saccharomyces pastorianus) |
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| Samples (12)
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| GSM8329259 |
SpIB1 lager yeast, day 5, rep1 |
| GSM8329260 |
SpIB1 lager yeast, day 5, rep2 |
| GSM8329261 |
SpIB1 lager yeast, day 5, rep3 |
| GSM8329262 |
SpIB2 lager yeast, day 2, rep1 |
| GSM8329263 |
SpIB2 lager yeast, day 2, rep2 |
| GSM8329264 |
SpIB2 lager yeast, day 2, rep3 |
| GSM8329265 |
SpIB2 lager yeast, day 5, rep1 |
| GSM8329266 |
SpIB2 lager yeast, day 5, rep2 |
| GSM8329267 |
SpIB2 lager yeast, day 5, rep3 |
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| Relations |
| BioProject |
PRJNA1124045 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269873_RAW.tar |
590.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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