 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 17, 2024 |
| Title |
The Hydractinia cell atlas reveals cellular and molecular principles of cnidarian coloniality |
| Organism |
Hydractinia symbiolongicarpus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used scRNA-seq to profile 199113 single cell transcriptomes of Hydractinia symbiolongicarpus, a highly regenerative colonial cnidarian. We characterized all major cell types, their transcription factors and gene networks, and analysed the basic mechanisms of coloniality in cnidarians.
|
| |
|
| Overall design |
Tissue (polyps or stolons) were isolated from a whole colony of male clone 291-10 by cutting. Samples were simultaneously fixed and dissociated using ACME. Colony parts are identified based on library and Round 1 barcode.
|
| |
|
| Contributor(s) |
Salamanca-Díaz DA, Horkan HR, García-Castro H, Emili E, Salinas-Saavedra M, Rossi E, Álvarez-Presas M, Mac Gabhann R, marsa F, Pérez-Posada A, Kenny NJ, Paps J, Frank U, Solana J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 14, 2024 |
| Last update date |
Jun 17, 2024 |
| Contact name |
Jordi Solana |
| E-mail(s) |
scbe.lab@gmail.com
|
| Organization name |
University of Exeter
|
| Department |
Health and Life Sciences
|
| Street address |
Northcote House
|
| City |
Exeter |
| ZIP/Postal code |
EX4 4QD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL34600 |
Illumina NovaSeq 6000 (Hydractinia symbiolongicarpus) |
|
| Samples (4)
|
| GSM8330343 |
Hydractinia symbiolongicarpus, whole-body, scRNA-seq, batch 1, library 9 |
| GSM8330344 |
Hydractinia symbiolongicarpus, whole-body, scRNA-seq, batch 1, library 20 |
| GSM8330345 |
Hydractinia symbiolongicarpus, whole-body, scRNA-seq, batch 1, library 27 |
| GSM8330346 |
Hydractinia symbiolongicarpus, whole-body, scRNA-seq, batch 1, library 29 |
|
| Relations |
| BioProject |
PRJNA1124116 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269914_barcodes.tsv.gz |
1.5 Mb |
(ftp)(http) |
TSV |
| GSE269914_features.tsv.gz |
88.8 Kb |
(ftp)(http) |
TSV |
| GSE269914_matrix.mtx.gz |
253.8 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...