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| Status |
Public on Jun 23, 2024 |
| Title |
Spatial Transcriptome Assays Reveals Gliomas Heterogeneity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: Glioma is a kind of highly heterogeneous central nervous system malignancy and controlled by various molecular processes such as neoplastic transformation, dysregulation of the cell cycle, and angiogenesis. Among these biomolecular events, the existence of inflammation and stress pathways in the development and driving factors of glioma heterogeneity has been reported. However, mechanisms of glioma heterogeneous under stress response remain unclear, especially from a spatial aspect. Methods: This study combined single-cell and spatially resolved transcriptomics and revealed that oxidative stress response genes play a vital role in oligodendrocyte precursor cells from two different types of gliomas: high- and low-grade (HG and LG). Results: In HG, stress triggers metabolic pattern changes from oxidative phosphorylation to glycolysis to avoid apoptosis, along with epithelial-to-mesenchymal transition and increased expression of genes of stress response. Scenic analysis indicated that oxidative stress induced the activation of AP1 in HG, thus enhancing the tumor survival and proliferation process. Conclusion: When all of these factors are considered together, we provide a unique perspective on how oxidative stress response occurs in different grades of gliomas, which would deepen our understanding of evolution and heterogeneity in gliomas.
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| Overall design |
Our primary glioma samples were obtained from untreated patients and analyzed using single-cell spatial transcriptomics profiling
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| Contributor(s) |
He Z, Wang Y, Song S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 20, 2024 |
| Last update date |
Jun 24, 2024 |
| Contact name |
Yi Wang |
| E-mail(s) |
w_yi@yahoo.com
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| Phone |
+8617708131072
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| Organization name |
Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital
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| Street address |
32 West Frist Ring Rd.,
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| City |
Chengdu |
| State/province |
Please Select |
| ZIP/Postal code |
610072 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1126248 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE270355_RAW.tar |
220.8 Mb |
(http)(custom) |
TAR (of PNG, TAR) |
SRA Run Selector |
| Raw data are available in SRA |
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